Matsumoto, T and Ingham, SM and Mifune, Y and Osawa, A and Logar, A and Usas, A and Kuroda, R and Kurosaka, M and Fu, FH and Huard, J
(2012)
Isolation and characterization of human anterior cruciate ligament-derived vascular stem cells.
Stem Cells and Development, 21 (6).
859 - 872.
ISSN 1547-3287
Abstract
The anterior cruciate ligament (ACL) usually fails to heal after rupture mainly due to the inability of the cells within the ACL tissue to establish an adequate healing process, making graft reconstruction surgery a necessity. However, some reports have shown that there is a healing potential of ACL with primary suture repair. Although some reports showed the existence of mesenchymal stem cell-like cells in human ACL tissues, their origin still remains unclear. Recently, blood vessels have been reported to represent a rich supply of stem/progenitor cells with a characteristic expression of CD34 and CD146. In this study, we attempted to validate the hypothesis that CD34- and CD146-expressing vascular cells exist in hACL tissues, have a potential for multi-lineage differentiation, and are recruited to the rupture site to participate in the intrinsic healing of injured ACL. Immunohistochemistry and flow cytometry analysis of hACL tissues demonstrated that it contains significantly more CD34 and CD146-positive cells in the ACL ruptured site compared with the noninjured midsubstance. CD34+CD45- cells isolated from ACL ruptured site showed higher expansionary potentials than CD146+CD45- and CD34-CD146-CD45- cells, and displayed higher differentiation potentials into osteogenic, adipogenic, and angiogenic lineages than the other cell populations. Immunohistochemistry of fetal and adult hACL tissues demonstrated a higher number of CD34 and CD146-positive cells in the ACL septum region compared with the midsubstance. In conclusion, our findings suggest that the ACL septum region contains a population of vascular-derived stem cells that may contribute to ligament regeneration and repair at the site of rupture. © 2012 Mary Ann Liebert, Inc.
Share
| Citation/Export: |
|
| Social Networking: |
|
Details
| Item Type: |
Article
|
| Status: |
Published |
| Creators/Authors: |
| Creators | Email | Pitt Username | ORCID  |
|---|
| Matsumoto, T | | | | | Ingham, SM | | | | | Mifune, Y | | | | | Osawa, A | | | | | Logar, A | | | | | Usas, A | | | | | Kuroda, R | | | | | Kurosaka, M | | | | | Fu, FH | ffu@pitt.edu | FFU | | | Huard, J | | | |
|
| Centers: |
Other Centers, Institutes, Offices, or Units > Stem Cell Research Center |
| Date: |
10 April 2012 |
| Date Type: |
Publication |
| Journal or Publication Title: |
Stem Cells and Development |
| Volume: |
21 |
| Number: |
6 |
| Page Range: |
859 - 872 |
| DOI or Unique Handle: |
10.1089/scd.2010.0528 |
| Schools and Programs: |
School of Medicine > Biochemistry and Molecular Genetics
School of Medicine > Orthopaedic Surgery
Swanson School of Engineering > Bioengineering |
| Refereed: |
Yes |
| ISSN: |
1547-3287 |
| MeSH Headings: |
Anterior Cruciate Ligament--blood supply; Antigens, CD146--analysis; Antigens, CD34--analysis; Blood Vessels--cytology; Cell Differentiation; Flow Cytometry; Humans; Immunohistochemistry; Stem Cells--cytology; Wound Healing |
| Other ID: |
NLM PMC3871494 |
| PubMed Central ID: |
PMC3871494 |
| PubMed ID: |
21732814 |
| Date Deposited: |
04 Apr 2014 15:41 |
| Last Modified: |
20 Jan 2019 10:55 |
| URI: |
http://d-scholarship.pitt.edu/id/eprint/20847 |
Metrics
Monthly Views for the past 3 years
Plum Analytics
Altmetric.com
Actions (login required)
 |
View Item |
![[img]](http://d-scholarship.pitt.edu/style/images/fileicons/text_plain.png)
![[feed]](/images/feed-icon-32x32.png){kind=icon}