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Development of a chemically defined medium and discovery of new mitogenic growth factors for mouse hepatocytes: Mitogenic effects of FGF1/2 and PDGF

Bowen, WC and Michalopoulos, AW and Orr, A and Ding, MQ and Stolz, DB and Michalopoulos, GK (2014) Development of a chemically defined medium and discovery of new mitogenic growth factors for mouse hepatocytes: Mitogenic effects of FGF1/2 and PDGF. PLoS ONE, 9 (4).

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Abstract

Chemically defined serum-free media for rat hepatocytes have been useful in identifying EGFR ligands and HGF/MET signaling as direct mitogenic factors for rat hepatocytes. The absence of such media for mouse hepatocytes has prevented screening for discovery of such mitogens for mouse hepatocytes. We present results obtained by designing such a chemically defined medium for mouse hepatocytes and demonstrate that in addition to EGFR ligands and HGF, the growth factors FGF1 and FGF2 are also important mitogenic factors for mouse hepatocytes. Smaller mitogenic response was also noticed for PDGF AB. Mouse hepatocytes are more likely to enter into spontaneous proliferation in primary culture due to activation of cell cycle pathways resulting from collagenase perfusion. These results demonstrate unanticipated fundamental differences in growth biology of hepatocytes between the two rodent species. Copyright: © 2014 Reekie et al.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Bowen, WCbowen@pitt.eduBOWEN
Michalopoulos, AW
Orr, Aavo4@pitt.eduAVO4
Ding, MQdingm@pitt.eduDINGM
Stolz, DBdonna.stolz@pitt.eduDSTOLZ
Michalopoulos, GKmichal@pitt.eduMICHAL
Contributors:
ContributionContributors NameEmailPitt UsernameORCID
EditorFacchiano, AntonioUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Date: 17 April 2014
Date Type: Publication
Journal or Publication Title: PLoS ONE
Volume: 9
Number: 4
DOI or Unique Handle: 10.1371/journal.pone.0095487
Schools and Programs: School of Medicine > Cell Biology
School of Medicine > Pathology
Refereed: Yes
Date Deposited: 23 Jun 2014 21:04
Last Modified: 02 Feb 2019 16:56
URI: http://d-scholarship.pitt.edu/id/eprint/21984

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