Comparative genomics of Cluster O mycobacteriophages
Cresawn, SG and Pope, WH and Jacobs-Sera, D and Bowman, CA and Russell, DA and Dedrick, RM and Adair, T and Anders, KR and Ball, S and Bollivar, D and Breitenberger, C and Burnett, SH and Butela, K and Byrnes, D and Carzo, S and Cornely, KA and Cross, T and Daniels, RL and Dunbar, D and Findley, AM and Gissendanner, CR and Golebiewska, UP and Hartzog, GA and Hatherill, JR and Hughes, LE and Jalloh, CS and De Los Santos, C and Ekanem, K and Khambule, SL and King, RA and King-Smith, C and Klyczek, K and Krukonis, GP and Laing, C and Lapin, JS and Lopez, AJ and Mkhwanazi, SM and Molloy, SD and Moran, D and Munsamy, V and Pacey, E and Plymale, R and Poxleitner, M and Reyna, N and Schildbach, JF and Stukey, J and Taylor, SE and Ware, VC and Wellmann, AL and Westholm, D and Wodarski, D and Zajko, M and Zikalala, TS and Hendrix, RW and Hatfull, GF
(2015)
Comparative genomics of Cluster O mycobacteriophages.
PLoS ONE, 10 (3).
Abstract
Mycobacteriophages - viruses of mycobacterial hosts - are genetically diverse but morphologically are all classified in the Caudovirales with double-stranded DNA and tails. We describe here a group of five closely related mycobacteriophages - Corndog, Catdawg, Dylan, Firecracker, and YungJamal - designated as Cluster O with long flexible tails but with unusual prolate capsids. Proteomic analysis of phage Corndog particles, Catdawg particles, and Corndog-infected cells confirms expression of half of the predicted gene products and indicates a non-canonical mechanism for translation of the Corndog tape measure protein. Bioinformatic analysis identifies 8-9 strongly predicted SigA promoters and all five Cluster O genomes contain more than 30 copies of a 17 bp repeat sequence with dyad symmetry located throughout the genomes. Comparison of the Cluster O phages provides insights into phage genome evolution including the processes of gene flux by horizontal genetic exchange.
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Creators | Email | Pitt Username | ORCID |
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Cresawn, SG | | | | Pope, WH | | | | Jacobs-Sera, D | djs@pitt.edu | DJS | 0000-0001-7838-3257 | Bowman, CA | | | | Russell, DA | dar78@pitt.edu | DAR78 | 0000-0001-6894-2748 | Dedrick, RM | dedrick@pitt.edu | DEDRICK | | Adair, T | | | | Anders, KR | | | | Ball, S | | | | Bollivar, D | | | | Breitenberger, C | | | | Burnett, SH | | | | Butela, K | KAB340@pitt.edu | KAB340 | | Byrnes, D | | | | Carzo, S | | | | Cornely, KA | | | | Cross, T | | | | Daniels, RL | | | | Dunbar, D | | | | Findley, AM | | | | Gissendanner, CR | | | | Golebiewska, UP | | | | Hartzog, GA | | | | Hatherill, JR | | | | Hughes, LE | | | | Jalloh, CS | | | | De Los Santos, C | | | | Ekanem, K | | | | Khambule, SL | | | | King, RA | | | | King-Smith, C | | | | Klyczek, K | | | | Krukonis, GP | | | | Laing, C | | | | Lapin, JS | | | | Lopez, AJ | | | | Mkhwanazi, SM | | | | Molloy, SD | | | | Moran, D | | | | Munsamy, V | | | | Pacey, E | | | | Plymale, R | | | | Poxleitner, M | | | | Reyna, N | | | | Schildbach, JF | | | | Stukey, J | | | | Taylor, SE | | | | Ware, VC | | | | Wellmann, AL | | | | Westholm, D | | | | Wodarski, D | | | | Zajko, M | | | | Zikalala, TS | | | | Hendrix, RW | rhx@pitt.edu | RHX | | Hatfull, GF | gfh@pitt.edu | GFH | |
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Contributors: |
Contribution | Contributors Name | Email | Pitt Username | ORCID |
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Editor | van Raaij, Mark J. | UNSPECIFIED | UNSPECIFIED | UNSPECIFIED |
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Date: |
5 March 2015 |
Date Type: |
Publication |
Journal or Publication Title: |
PLoS ONE |
Volume: |
10 |
Number: |
3 |
DOI or Unique Handle: |
10.1371/journal.pone.0118725 |
Schools and Programs: |
Dietrich School of Arts and Sciences > Biological Sciences |
Refereed: |
Yes |
Article Type: |
Review |
PubMed ID: |
25742016 |
Date Deposited: |
12 May 2015 18:06 |
Last Modified: |
30 Mar 2021 10:55 |
URI: |
http://d-scholarship.pitt.edu/id/eprint/24099 |
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