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Two-stage therapeutic utility of ectopically formed bone tissue in skeletal muscle induced by adeno-associated virus containing bone morphogenetic protein-4 gene

Tian, K and Qi, M and Wang, L and Li, Z and Xu, J and Li, Y and Liu, G and Wang, B and Huard, J and Li, G (2015) Two-stage therapeutic utility of ectopically formed bone tissue in skeletal muscle induced by adeno-associated virus containing bone morphogenetic protein-4 gene. Journal of Orthopaedic Surgery and Research, 10 (1).

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Abstract

Background: The major disadvantage of using a stem cell-based bone morphogenetic protein-4 (BMP4) gene therapy for skull defect is the overgrowth of generated bone tissue in situ. In the present study, to overcome bony overgrowth of stem cell based-gene therapy, a new strategy of two-stage bone tissue engineering by an adeno-associated virus containing BMP4 gene (AAV-BMP4) gene therapy was used. Methods: AAV-BMP4 was purposely implanted in the skeletal muscle of mice to generate ectopic bone tissues during the first stage. Next, the newly formed ectopic bone tissues were harvested and then transplanted to repair the mouse skull defect during the second stage. Results: The results showed that skeletal muscle implantation of AAV-BMP4 yielded a large amount of new bone tissues. The ectopic bone tissues can be harvested as a bone graft and can successfully repair the mouse skull defect without any bony overgrowth in situ. Conclusion: The results indicate that the bone tissues purposely generated by AAV-BMP4 in the skeletal muscle may be a new alternative of bone grafting for clinical purposes.

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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Tian, K
Qi, M
Wang, L
Li, Z
Xu, J
Li, Y
Liu, G
Wang, Bbingwang@pitt.eduBINGWANG
Huard, J
Li, G
Centers: Other Centers, Institutes, Offices, or Units > Stem Cell Research Center
Date: 30 May 2015
Date Type: Publication
Journal or Publication Title: Journal of Orthopaedic Surgery and Research
Volume: 10
Number: 1
DOI or Unique Handle: 10.1186/s13018-015-0229-1
Schools and Programs: School of Medicine > Orthopaedic Surgery
Refereed: Yes
Date Deposited: 16 Aug 2016 16:49
Last Modified: 30 Mar 2021 13:55
URI: http://d-scholarship.pitt.edu/id/eprint/29268

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