Link to the University of Pittsburgh Homepage
Link to the University Library System Homepage Link to the Contact Us Form

Immunohistochemical staining of radixin and moesin in prostatic adenocarcinoma

Bartholow, TL and Chandran, UR and Becich, MJ and Parwani, AV (2011) Immunohistochemical staining of radixin and moesin in prostatic adenocarcinoma. BMC Clinical Pathology, 11. ISSN 1472-6890

[img]
Preview
PDF
Published Version
Available under License : See the attached license file.

Download (1MB) | Preview
[img] Plain Text (licence)
Available under License : See the attached license file.

Download (1kB)

Abstract

Abstract. Background. Some members of the Protein 4.1 superfamily are believed to be involved in cell proliferation and growth, or in the regulation of these processes. While the expression levels of two members of this family, radixin and moesin, have been studied in many tumor types, to our knowledge they have not been investigated in prostate cancer. Methods. Tissue microarrays were immunohistochemically stained for either radixin or moesin, with the staining intensities subsequently quantified and statistically analyzed using One-Way ANOVA or nonparametric equivalent with subsequent Student-Newman-Keuls tests for multiple comparisons. There were 11 cases of normal donor prostates (NDP), 14 cases of benign prostatic hyperplasia (BPH), 23 cases of high-grade prostatic intraepithelial neoplasia (HGPIN), 88 cases of prostatic adenocarcinoma (PCa), and 25 cases of normal tissue adjacent to adenocarcinoma (NAC) analyzed in the microarrays. Results. NDP, BPH, and HGPIN had higher absolute staining scores for radixin than PCa and NAC, but with a significant difference observed between only HGPIN and PCa (p = < 0.001) and HGPIN and NAC (p = 0.001). In the moesin-stained specimens, PCa, NAC, HGPIN, and BPH all received absolute higher staining scores than NDP, but the differences were not significant. Stage 4 moesin-stained PCa had a significantly reduced staining intensity compared to Stage 2 (p = 0.003). Conclusions. To our knowledge, these studies represent the first reports on the expression profiles of radixin and moesin in prostatic adenocarcinoma. The current study has shown that there were statistically significant differences observed between HGPIN and PCa and HGPIN and NAC in terms of radixin expression. The differences in the moesin profiles by tissue type were not statistically significant. Additional larger studies with these markers may further elucidate their potential roles in prostatic neoplasia progression. © 2011 Bartholow et al; licensee BioMed Central Ltd.


Share

Citation/Export:
Social Networking:
Share |

Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Bartholow, TL
Chandran, URchandran@pitt.eduCHANDRAN
Becich, MJbecich@pitt.eduBECICH
Parwani, AV
Date: 18 January 2011
Date Type: Publication
Journal or Publication Title: BMC Clinical Pathology
Volume: 11
DOI or Unique Handle: 10.1186/1472-6890-11-1
Schools and Programs: School of Medicine > Biomedical Informatics
School of Medicine > Pathology
Refereed: Yes
ISSN: 1472-6890
Date Deposited: 16 Nov 2016 18:48
Last Modified: 02 Feb 2019 16:56
URI: http://d-scholarship.pitt.edu/id/eprint/30193

Metrics

Monthly Views for the past 3 years

Plum Analytics

Altmetric.com


Actions (login required)

View Item View Item