Smethurst, Victoria
(2018)
Role of Inorganic Phosphate in Vesicle Release from Osteogenic Cells.
Master's Thesis, University of Pittsburgh.
(Unpublished)
This is the latest version of this item.
Abstract
Extracellular vesicles (EV) are membrane-enclosed microstructures released from the cell. EV can transport cargo—such as DNA, RNA, and proteins—into the extracellular space and act as a form of intercellular communication. Matrix vesicles (MV) are a unique type of EV produced by mineralizing cells. In previous studies, utilizing in vitro models of pre-odontoblasts and vascular smooth muscle cells, it was determined that phosphate induces the release of MV and alters MV composition. We hypothesized that elevated phosphate alters the composition of MV through the alteration of gene expression. To determine whether this hypothesis holds true or not, 17IIA11 cells, a pre-odontoblast cell line, were exposed to elevated phosphate for 24h, 48h, and 72h. RNA and MV were then collected for further analysis. Using nanoparticle tracking analysis, we determined that the size distribution of the MV was not altered by the phosphate. Next, to evaluate a possible mechanism driving MV composition change, we compared the effect of phosphate on gene expression to the effect of phosphate on MV composition. No correlation was identified between changes in gene expression and changes in MV composition. These findings suggest that phosphate does not alter the composition of MV through altered gene expression. Future studies should focus on other mechanisms that may drive MV composition changes.
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Details
| Item Type: |
University of Pittsburgh ETD
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| Status: |
Unpublished |
| Creators/Authors: |
| Creators | Email | Pitt Username | ORCID  |
|---|
| Smethurst, Victoria | ves33@pitt.edu | ves33 | |
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| ETD Committee: |
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| Date: |
29 August 2018 |
| Date Type: |
Publication |
| Defense Date: |
19 July 2018 |
| Approval Date: |
29 August 2018 |
| Submission Date: |
17 August 2018 |
| Access Restriction: |
2 year -- Restrict access to University of Pittsburgh for a period of 2 years. |
| Number of Pages: |
59 |
| Institution: |
University of Pittsburgh |
| Schools and Programs: |
School of Dental Medicine > Dental Science |
| Degree: |
MS - Master of Science |
| Thesis Type: |
Master's Thesis |
| Refereed: |
Yes |
| Uncontrolled Keywords: |
N/A |
| Date Deposited: |
29 Aug 2018 14:37 |
| Last Modified: |
29 Aug 2020 05:15 |
| URI: |
http://d-scholarship.pitt.edu/id/eprint/35252 |
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Role of Inorganic Phosphate in Vesicle Release from Osteogenic Cells. (deposited 29 Aug 2018 14:37)
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