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A new liver perfusion and preservation system for transplantation Research in large animals

Yanaga, K and Makowka, L and Lebeau, G and Hwang, RR and Shimada, M and Kakizoe, S and Demetris, AJ and Starzl, TE (1990) A new liver perfusion and preservation system for transplantation Research in large animals. Journal of Investigative Surgery, 3 (1). 65 - 75. ISSN 0894-1939

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Abstract

A kidney perfusion machine, model MOX-100 (Waters Instruments, Ltd, Rochester, MN) was modified to allow continuous perfusion of the portal vein and pulsatile perfusion of the hepatic artery of the liver. Additional apparatus consists of a cooling system, a membrane oxygenator, a filter for foreign bodies, and bubble traps. This system not only allows hypothermic perfusion preservation of the liver graft, but furthermore enables investigation of ex vivo simulation of various circulatory circumstances in which physiological perfusion of the liver is studied. We have used this system to evaluate the viability of liver allografts preserved by cold storage. The liver was placed on the perfusion system and perfused with blood with a hematocrit of approximately 20% and maintained at 37°C for 3 h. The flows of the hepatic artery and portal vein were adjusted to 0.33 mL and 0.67 mL/g of liver tissue, respectively. Parameters of viability consisted of hourly bile output, oxygen consumption, liver enzymes, electrolytes, vascular resistance, and liver histology. This method of liver assessment in large animals will allow the objective evaluation of organ viability for transplantation and thereby improve the outcome of organ transplantation. Furthermore, this pump enables investigation into the pathophysiology of liver ischemia and preservation. © 1990 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Yanaga, K
Makowka, L
Lebeau, G
Hwang, RR
Shimada, M
Kakizoe, S
Demetris, AJ
Starzl, TEtes11@pitt.eduTES11
Centers: Other Centers, Institutes, Offices, or Units > Thomas E. Starzl Transplantation Institute
Date: 1 January 1990
Date Type: Publication
Journal or Publication Title: Journal of Investigative Surgery
Volume: 3
Number: 1
Page Range: 65 - 75
DOI or Unique Handle: 10.3109/08941939009140337
Institution: University of Pittsburgh
Refereed: Yes
ISSN: 0894-1939
Other ID: uls-drl:31735062110832, Starzl CV No. 1041
Date Deposited: 08 Apr 2010 17:17
Last Modified: 27 Jan 2019 02:55
URI: http://d-scholarship.pitt.edu/id/eprint/4427

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