Demetris, AJ and Qian, S and Sun, H and Fung, JJ and Yagihashi, A and Murase, N and Iwaki, Y and Gambrell, B and Starzl, TE
(1991)
Early events in liver allograft rejection. Delineation of sites of simultaneous intragraft and recipient lymphoid tissue sensitization.
American Journal of Pathology, 138 (3).
609 - 618.
ISSN 0002-9440
Abstract
The early events of liver allograft rejection in untreated rats were studied in the DA to BN rejection strain combination and compared with DA and BN liver isograft recipients. In the liver allografts, T-cell infiltration first occurred at 2 days after transplantation and localized to the portal triads and subjacent to the terminal hepatic venules (THV), regions rich in intensely Ia+ spindle and dendritic-shaped interstitial cells. Double staining showed distinct 'clustering' between donor Ia-positive dendritic shaped cells and W3/25+ infiltrating lymphocytes, or to a lesser extent, 0X8+ cells. The infiltrating mononuclear cells underwent blastogenesis and proliferated in both the triads and THV regions at 3 and 4 days. Donor Ia-positive cells were also noted in the W3/25+ periarterial lymphatic sheath and marginal zone of the recipient spleen 1 day after transplantation. The number of these cells in the spleen peaked at 3 to 4 days, but were no longer detectable by 10 to 12 days. Mitotic activity became evident in these same regions by days 3 and 4. Paracortical blastogenesis (day 2) and proliferation (days 3 and 4) were also noted in the regional lymph nodes of liver allograft recipients, but no donor Ia+ cells were found in the mesenteric nodes or thymus of the allograft recipients. These results demonstrate that sensitization of the recipient lymphoid tissue to liver allografts can occur both peripherally (intragraft) and centrally (spleen and lymph nodes). Passenger leukocytes (donor dendritic cells) are likely the primary stimulators of the rejection reaction. Still, it is probable that other pathways of sensitization exist.
Share
| Citation/Export: |
|
| Social Networking: |
|
Details
| Item Type: |
Article
|
| Status: |
Published |
| Creators/Authors: |
| Creators | Email | Pitt Username | ORCID  |
|---|
| Demetris, AJ | | | | | Qian, S | | | | | Sun, H | | | | | Fung, JJ | | | | | Yagihashi, A | | | | | Murase, N | | | | | Iwaki, Y | | | | | Gambrell, B | | | | | Starzl, TE | tes11@pitt.edu | TES11 | |
|
| Centers: |
Other Centers, Institutes, Offices, or Units > Thomas E. Starzl Transplantation Institute |
| Date: |
1 January 1991 |
| Date Type: |
Publication |
| Journal or Publication Title: |
American Journal of Pathology |
| Volume: |
138 |
| Number: |
3 |
| Page Range: |
609 - 618 |
| Institution: |
University of Pittsburgh |
| Refereed: |
Yes |
| ISSN: |
0002-9440 |
| Other ID: |
uls-drl:31735062112739, Starzl CV No. 1486 |
| Date Deposited: |
08 Apr 2010 17:25 |
| Last Modified: |
17 Dec 2017 02:55 |
| URI: |
http://d-scholarship.pitt.edu/id/eprint/4872 |
Metrics
Monthly Views for the past 3 years
Plum Analytics
Actions (login required)
 |
View Item |
![[img]](http://d-scholarship.pitt.edu/style/images/fileicons/text_plain.png)
![[feed]](/images/feed-icon-32x32.png){kind=icon}