Cloning and sequence analysis of the rat augmenter of liver regeneration (ALR) gene: Expression of biologically active recombinant ALR and demonstration of tissue distribution

Hagiya, M and Francavilla, A and Polimeno, L and Ihara, I and Sakai, H and Seki, T and Shimonishi, M and Porter, KA and Starzl, TE (1994) Cloning and sequence analysis of the rat augmenter of liver regeneration (ALR) gene: Expression of biologically active recombinant ALR and demonstration of tissue distribution. Proceedings of the National Academy of Sciences of the United States of America, 91 (17). 8142 - 8146. ISSN 0027-8424

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Abstract

A full-length cDNA clone encoding a purified augmenter of liver regeneration (ALR) factor prepared from the cytosol of weanling rat livers was isolated. The 1.2-kb cDNA included a 299-bp 5' untranslated region, a 375-bp coding region, and a 550-bp 3' untranslated region. It encoded a protein consisting of 125 amino acids. The molecular weight of ALR calculated from the cDNA was 15,081, which is consistent with the size estimated by SDS/PAGE under reducing conditions. The molecular weight of the purified native ALR estimated by SDS/PAGE under nonreducing conditions was ≃30,000; thus ALR apparently has a homodimeric structure. The recombinant ALR produced by expression of the cDNA in COS cells was tested in vivo in the canine Eck fistula model and found to have potency equivalent to the purified native ALR. The 125-aa sequence deduced from the rat ALR cDNA shows 50% homology to the amino acid sequence of the gene for oxidative phosphorylation and vegetative growth in the yeast Saccharomyces cerevisiae.

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Details

Item Type:	Article
Status:	Published
Creators/Authors:	Creators Email Pitt Username ORCID Hagiya, M Francavilla, A Polimeno, L Ihara, I Sakai, H Seki, T Shimonishi, M Porter, KA Starzl, TE tes11@pitt.edu TES11
Centers:	Other Centers, Institutes, Offices, or Units > Thomas E. Starzl Transplantation Institute
Date:	16 August 1994
Date Type:	Publication
Journal or Publication Title:	Proceedings of the National Academy of Sciences of the United States of America
Volume:	91
Number:	17
Page Range:	8142 - 8146
DOI or Unique Handle:	10.1073/pnas.91.17.8142
Institution:	University of Pittsburgh
Refereed:	Yes
ISSN:	0027-8424
Other ID:	uls-drl:31735062124734, Starzl CV No. 1635
Date Deposited:	08 Apr 2010 17:27
Last Modified:	02 Feb 2019 13:55
URI:	http://d-scholarship.pitt.edu/id/eprint/5021

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