Todo, S and Hamada, N and Zhu, Y and Zhang, S and Subbotin, V and Nemoto, A and Takeyoshi, I and Starzl, TE
(1996)
Lazaroid U-74389G for 48-hour canine liver preservation.
Transplantation, 61 (2).
189 - 194.
ISSN 0041-1337
Abstract
Lazaroids have been reported to attenuate preservation and reperfusion injury. In this study, we examined whether lazaroids can improve the outcome after 48-hr canine liver preservation and transplantation. Adult female beagle dogs were randomized into 4 dosage groups (5 animals each). Lazaroid U-74389G was intravenously administered at a dose of 0 mg/kg, 6 mg/kg, 10 mg/kg, or 15 mg/kg to donors 30 min before harvesting and also to recipients 30 min before revascularization. Control animals (0 mg/kg) were given the lazaroid vehicle. The liver grafts were orthotopically transplanted after 48 hr of hypothermic preservation in UW solution. Lazaroid treatment significantly improved outcome after transplantation. Five-day animal survival increased from 0% in the control to 60% in the 6 mg/kg group, 100% in the 10 mg/kg group, and 80% in the 15 mg/kg group. Lazaroid protected the hepatocytes from damage during preservation, and enhanced energy charge and hepatic blood flow after reperfusion. Histological alterations were significantly less severe in the lazaroid-treated groups. The area of necrotic hepatocytes decreased from 43.7±17.7 in the control to 13.5±3.0 in the lazaroid 10 mg/kg group. These results indicate that lazaroid U-74389G has potential for improvement of clinical liver preservation.
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Item Type: |
Article
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Status: |
Published |
Creators/Authors: |
Creators | Email | Pitt Username | ORCID |
---|
Todo, S | | | | Hamada, N | | | | Zhu, Y | | | | Zhang, S | | | | Subbotin, V | | | | Nemoto, A | | | | Takeyoshi, I | | | | Starzl, TE | tes11@pitt.edu | TES11 | |
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Centers: |
Other Centers, Institutes, Offices, or Units > Thomas E. Starzl Transplantation Institute |
Date: |
27 January 1996 |
Date Type: |
Publication |
Journal or Publication Title: |
Transplantation |
Volume: |
61 |
Number: |
2 |
Page Range: |
189 - 194 |
DOI or Unique Handle: |
10.1097/00007890-199601270-00004 |
Institution: |
University of Pittsburgh |
Refereed: |
Yes |
ISSN: |
0041-1337 |
Other ID: |
uls-drl:31735062121714, Starzl CV No. 1819 |
Date Deposited: |
08 Apr 2010 17:31 |
Last Modified: |
02 Feb 2019 13:57 |
URI: |
http://d-scholarship.pitt.edu/id/eprint/5205 |
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