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An exploratory pathways analysis of temporal changes induced by spinal cord injury in the Rat bladder wall: Insights on remodeling and inflammation

Wognum, S and Lagoa, CE and Nagatomi, J and Sacks, MS and Vodovotz, Y (2009) An exploratory pathways analysis of temporal changes induced by spinal cord injury in the Rat bladder wall: Insights on remodeling and inflammation. PLoS ONE, 4 (6).

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Abstract

Background: Spinal cord injuries (SCI) can lead to severe bladder pathologies associated with inflammation, fibrosis, and increased susceptibility to urinary tract infections. We sought to characterize the complex pathways of remodeling, inflammation, and infection in the urinary bladder at the level of the transcriptome in a rat model of SCI, using pathways analysis bioinformatics. Methodology/Principal Findings: Experimental data were obtained from the study of Nagatomi et al. (Biochem Biophys Res Commun 334: 1159). In this study, bladders from rats subjected to surgical SCI were obtained at 3, 7 or 25 days post-surgery, and Affymetrix GeneChip® Rat Genome U34A arrays were used for cRNA hybridizations. In the present study, Ingenuity Pathways Analysis (Ingenuity® Systems, www.ingenuity.com) of differentially expressed genes was performed. Analysis of focus genes in networks, functional analysis, and canonical pathway analysis reinforced our previous findings related to the presence of up-regulated genes involved in tissue remodeling, such as lysyl oxidase, tropoelastin, TGF-β1, and IGF-1. This analysis also highlighted a central role for inflammation and infection, evidenced by networks containing genes such as CD74, S100A9, and THY1. Conclusions/Significance: Our findings suggest that tissue remodeling, infection, inflammation, and tissue damage/ dysfunction all play a role in the urinary bladder, in the complex response to SCI. © 2009 Wognum et al.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Wognum, S
Lagoa, CE
Nagatomi, J
Sacks, MS
Vodovotz, Yvodovotz@pitt.eduVODOVOTZ
Contributors:
ContributionContributors NameEmailPitt UsernameORCID
EditorPeccoud, JeanUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Date: 9 June 2009
Date Type: Publication
Journal or Publication Title: PLoS ONE
Volume: 4
Number: 6
DOI or Unique Handle: 10.1371/journal.pone.0005852
Schools and Programs: Swanson School of Engineering > Bioengineering
Refereed: Yes
PubMed Central ID: PMC2688838
PubMed ID: 19513121
Date Deposited: 25 Jul 2012 14:21
Last Modified: 04 Feb 2019 15:58
URI: http://d-scholarship.pitt.edu/id/eprint/13144

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