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A trivalent virus-like particle vaccine elicits protective immune responses against seasonal influenza strains in mice and ferrets

Ross, TM and Mahmood, K and Crevar, CJ and Schneider-Ohrum, K and Heaton, PM and Bright, RA (2009) A trivalent virus-like particle vaccine elicits protective immune responses against seasonal influenza strains in mice and ferrets. PLoS ONE, 4 (6).

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Abstract

There is need for improved human influenza vaccines, particularly for older adults who are at greatest risk for severe disease, as well as to address the continuous antigenic drift within circulating human subtypes of influenza virus. We have engineered an influenza virus-like particle (VLP) as a new generation vaccine candidate purified from the supernatants of Sf9 insect cells following infection by recombinant baculoviruses to express three influenza virus proteins, hemagglutinin (HA), neuraminidase (NA), and matrix 1 (M1). In this study, a seasonal trivalent VLP vaccine (TVV) formulation, composed of influenza A H1N1 and H3N2 and influenza B VLPs, was evaluated in mice and ferrets for the ability to elicit antigen-specific immune responses. Animals vaccinated with the TVV formulation had hemagglutination-inhibition (HAI) antibody titers against all three homologous influenza virus strains, as well as HAI antibodies against a panel of heterologous influenza viruses. HAI titers elicited by the TVV were statistically similar to HAI titers elicited in animals vaccinated with the corresponding monovalent VLP. Mice vaccinated with the TVV had higher level of influenza specific CD8+ T cell responses than a commercial trivalent inactivated vaccine (TIV). Ferrets vaccinated with the highest dose of the VLP vaccine and then challenged with the homologous H3N2 virus had the lowest titers of replicating virus in nasal washes and showed no signs of disease. Overall, a trivalent VLP vaccine elicits a broad array of immunity and can protect against influenza virus challenge. © 2009 Ross et al.

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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Ross, TM
Mahmood, K
Crevar, CJ
Schneider-Ohrum, K
Heaton, PM
Bright, RA
Contributors:
ContributionContributors NameEmailPitt UsernameORCID
EditorLiu, Ding XiangUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Centers: Other Centers, Institutes, Offices, or Units > Center for Vaccine Research
Date: 24 June 2009
Date Type: Publication
Journal or Publication Title: PLoS ONE
Volume: 4
Number: 6
DOI or Unique Handle: 10.1371/journal.pone.0006032
Refereed: Yes
PubMed Central ID: PMC2698286
PubMed ID: 19554101
Date Deposited: 30 Jul 2012 22:09
Last Modified: 02 Feb 2019 16:57
URI: http://d-scholarship.pitt.edu/id/eprint/13148

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