Rohan, LC and Moncla, BJ and Kunjara Na Ayudhya, RP and Cost, M and Huang, Y and Gai, F and Billitto, N and Lynam, JD and Pryke, K and Graebing, P and Hopkins, N and Rooney, JF and Friend, D and Dezzutti, CS
(2010)
In vitro and ex vivo testing of tenofovir shows it is effective as an HIV-1 microbicide.
PLoS ONE, 5 (2).
Abstract
Background: Tenofovir gel has entered into clinical trials for use as a topical microbicide to prevent HIV-1 infection but has no published data regarding pre-clinical testing using in vitro and ex vivo models. To validate our findings with on-going clinical trial results, we evaluated topical tenofovir gel for safety and efficacy. We also modeled systemic application of tenofovir for efficacy. Methods and Findings: Formulation assessment of tenofovir gel included osmolality, viscosity, in vitro release, and permeability testing. Safety was evaluated by measuring the effect on the viability of vaginal flora, PBMCs, epithelial cells, and ectocervical and colorectal explant tissues. For efficacy testing, PBMCs were cultured with tenofovir or vehicle control gels and HIV-1 representing subtypes A, B, and C. Additionally, polarized ectocervical and colorectal explant cultures were treated apically with either gel. Tenofovir was added basolaterally to simulate systemic application. All tissues were challenged with HIV-1 applied apically. Infection was assessed by measuring p24 by ELISA on collected supernatants and immunohisto-chemistry for ectocervical explants. Formulation testing showed the tenofovir and vehicle control gels were >10 times isosmolar. Permeability through ectocervical tissue was variable but in all cases the receptor compartment drug concentration reached levels that inhibit HIV-1 infection in vitro. The gels were non-toxic toward vaginal flora, PBMCs, or epithelial cells. A transient reduction in epithelial monolayer integrity and epithelial fracture for ectocervical and colorectal explants was noted and likely due to the hyperosmolar nature of the formulation. Tenofovir gel prevented HIV-1 infection of PBMCs regardless of HIV-1 subtype. Topical and systemic tenofovir were effective at preventing HIV-1 infection of explant cultures. Conclusions: These studies provide a mechanism for pre-clinical prediction of safety and efficacy of formulated microbicides. Tenofovir was effective against HIV-1 infection in our algorithm. These data support the use of tenofovir for pre-exposure prophylaxis. © 2010 Rohan et al.
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Item Type: |
Article
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Status: |
Published |
Creators/Authors: |
Creators | Email | Pitt Username | ORCID  |
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Rohan, LC | rohanl@pitt.edu | ROHANL | | Moncla, BJ | bjm4@pitt.edu | BJM4 | | Kunjara Na Ayudhya, RP | | | | Cost, M | | | | Huang, Y | | | | Gai, F | | | | Billitto, N | | | | Lynam, JD | | | | Pryke, K | | | | Graebing, P | | | | Hopkins, N | | | | Rooney, JF | | | | Friend, D | | | | Dezzutti, CS | csd13@pitt.edu | CSD13 | |
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Contributors: |
Contribution | Contributors Name | Email | Pitt Username | ORCID  |
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Editor | Stoddart, Cheryl A. | UNSPECIFIED | UNSPECIFIED | UNSPECIFIED |
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Date: |
19 February 2010 |
Date Type: |
Publication |
Journal or Publication Title: |
PLoS ONE |
Volume: |
5 |
Number: |
2 |
DOI or Unique Handle: |
10.1371/journal.pone.0009310 |
Refereed: |
Yes |
MeSH Headings: |
Adenine--analogs & derivatives; Adenine--pharmacology; Anti-HIV Agents--pharmacology; Caco-2 Cells; Cell Survival--drug effects; Cells, Cultured; Cervix Uteri--cytology; Cervix Uteri--drug effects; Cervix Uteri--virology; Colon--cytology; Colon--drug effects; Colon--virology; Drug Evaluation, Preclinical; Female; Gels; HIV-1--drug effects; HIV-1--growth & development; Humans; Leukocytes, Mononuclear--cytology; Leukocytes, Mononuclear--drug effects; Leukocytes, Mononuclear--virology; Phosphonic Acids--pharmacology; Rectum--cytology; Rectum--drug effects; Rectum--virology; Tissue Culture Techniques |
Other ID: |
NLM PMC2824823 |
PubMed Central ID: |
PMC2824823 |
PubMed ID: |
20174579 |
Date Deposited: |
03 Aug 2012 18:47 |
Last Modified: |
03 Feb 2019 10:55 |
URI: |
http://d-scholarship.pitt.edu/id/eprint/13332 |
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