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Serological profiling of a Candida albicans protein microarray reveals permanent host-pathogen interplay and stage-specific responses during Candidemia

Brian Mochon, A and Ye, J and Kayala, MA and Wingard, JR and Clancy, CJ and Hong Nguyen, M and Felgner, P and Baldi, P and Liu, H (2010) Serological profiling of a Candida albicans protein microarray reveals permanent host-pathogen interplay and stage-specific responses during Candidemia. PLoS Pathogens, 6 (3). ISSN 1553-7366

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Abstract

Candida albicans in the immunocompetent host is a benign member of the human microbiota. Though, when host physiology is disrupted, this commensal-host interaction can degenerate and lead to an opportunistic infection. Relatively little is known regarding the dynamics of C. albicans colonization and pathogenesis. We developed a C. albicans cell surface protein microarray to profile the immunoglobulin G response during commensal colonization and candidemia. The antibody response from the sera of patients with candidemia and our negative control groups indicate that the immunocompetent host exists in permanent host-pathogen interplay with commensal C. albicans. This report also identifies cell surface antigens that are specific to different phases (i.e. acute, early and mid convalescence) of candidemia. We identified a set of thirteen cell surface antigens capable of distinguishing acute candidemia from healthy individuals and uninfected hospital patients with commensal colonization. Interestingly, a large proportion of these cell surface antigens are involved in either oxidative stress or drug resistance. In addition, we identified 33 antigenic proteins that are enriched in convalescent sera of the candidemia patients. Intriguingly, we found within this subset an increase in antigens associated with heme-associated iron acquisition. These findings have important implications for the mechanisms of C. albicans colonization as well as the development of systemic infection.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Brian Mochon, A
Ye, J
Kayala, MA
Wingard, JR
Clancy, CJcjc76@pitt.eduCJC76
Hong Nguyen, M
Felgner, P
Baldi, P
Liu, H
Contributors:
ContributionContributors NameEmailPitt UsernameORCID
EditorDoering, Tamara L.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Date: 1 March 2010
Date Type: Publication
Journal or Publication Title: PLoS Pathogens
Volume: 6
Number: 3
DOI or Unique Handle: 10.1371/journal.ppat.1000827
Refereed: Yes
ISSN: 1553-7366
MeSH Headings: Antibodies, Fungal--blood; Antigens, Fungal--immunology; Antigens, Fungal--metabolism; Biological Markers--blood; Candida albicans--growth & development; Candida albicans--immunology; Candidiasis--blood; Candidiasis--epidemiology; Candidiasis--immunology; Convalescence; Heme--metabolism; Host-Pathogen Interactions--immunology; Humans; Immunoglobulin G--blood; Protein Array Analysis; Seroepidemiologic Studies
Other ID: NLM PMC2845659
PubMed Central ID: PMC2845659
PubMed ID: 20361054
Date Deposited: 03 Aug 2012 18:55
Last Modified: 02 Feb 2019 16:58
URI: http://d-scholarship.pitt.edu/id/eprint/13344

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