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Study of muscle cell dedifferentiation after skeletal muscle injury of mice with a Cre-Lox system

Mu, X and Peng, H and Pan, H and Huard, J and Li, Y (2011) Study of muscle cell dedifferentiation after skeletal muscle injury of mice with a Cre-Lox system. PLoS ONE, 6 (2).

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Abstract

Background: Dedifferentiation of muscle cells in the tissue of mammals has yet to be observed. One of the challenges facing the study of skeletal muscle cell dedifferentiation is the availability of a reliable model that can confidentially distinguish differentiated cell populations of myotubes and non-fused mononuclear cells, including stem cells that can coexist within the population of cells being studied. Methodology/Principal Findings: In the current study, we created a Cre/Lox-β-galactosidase system, which can specifically tag differentiated multinuclear myotubes and myotube-generated mononuclear cells based on the activation of the marker gene, β-galactosidase. By using this system in an adult mouse model, we found that β-galactosidase positive mononuclear cells were generated from β-galactosidase positive multinuclear myofibers upon muscle injury. We also demonstrated that these mononuclear cells can develop into a variety of different muscle cell lineages, i.e., myoblasts, satellite cells, and muscle derived stem cells. Conclusions/Significance: These novel findings demonstrated, for the first time, that cellular dedifferentiation of skeletal muscle cells actually occurs in mammalian skeletal muscle following traumatic injury in vivo. © 2011 Mu et al.

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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Mu, X
Peng, H
Pan, H
Huard, J
Li, Y
Date: 14 February 2011
Date Type: Publication
Journal or Publication Title: PLoS ONE
Volume: 6
Number: 2
DOI or Unique Handle: 10.1371/journal.pone.0016699
Schools and Programs: Swanson School of Engineering > Bioengineering
Refereed: Yes
MeSH Headings: Animals; Cell Dedifferentiation--genetics; Cell Dedifferentiation--physiology; Cell Tracking--methods; Integrases--genetics; Integrases--metabolism; Male; Mice; Mice, Inbred C57BL; Mice, SCID; Mice, Transgenic; Muscle Fibers, Skeletal--metabolism; Muscle Fibers, Skeletal--pathology; Muscle Fibers, Skeletal--physiology; Muscle, Skeletal--injuries; Muscle, Skeletal--metabolism; Muscle, Skeletal--pathology; Organ Specificity--genetics; Regeneration--physiology; Transgenes--physiology; beta-Galactosidase--genetics; beta-Galactosidase--metabolism
Other ID: NLM PMC3033395
PubMed Central ID: PMC3033395
PubMed ID: 21304901
Date Deposited: 03 Aug 2012 19:00
Last Modified: 13 Oct 2017 22:56
URI: http://d-scholarship.pitt.edu/id/eprint/13354

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