Yang, F and Cheng, Y and An, JY and Kwon, YT and Eckardt, S and Leu, NA and Mclaughlin, KJ and Wang, PJ
(2010)
The ubiquitin ligase Ubr2, a recognition E3 component of the N-end rule pathway, stabilizes Tex19.1 during spermatogenesis.
PLoS ONE, 5 (11).
Abstract
Ubiquitin E3 ligases target their substrates for ubiquitination, leading to proteasome-mediated degradation or altered biochemical properties. The ubiquitin ligase Ubr2, a recognition E3 component of the N-end rule proteolytic pathway, recognizes proteins with N-terminal destabilizing residues and plays an important role in spermatogenesis. Tex19.1 (also known as Tex19) has been previously identified as a germ cell-specific protein in mouse testis. Here we report that Tex19.1 forms a stable protein complex with Ubr2 in mouse testes. The binding of Tex19.1 to Ubr2 is independent of the second position cysteine of Tex19.1, a putative target for arginylation by the N-end rule pathway R-transferase. The Tex19.1-null mouse mutant phenocopies the Ubr2-deficient mutant in three aspects: heterogeneity of spermatogenic defects, meiotic chromosomal asynapsis, and embryonic lethality preferentially affecting females. In Ubr2-deficient germ cells, Tex19.1 is transcribed, but Tex19.1 protein is absent. Our results suggest that the binding of Ubr2 to Tex19.1 metabolically stabilizes Tex19.1 during spermatogenesis, revealing a new function for Ubr2 outside the conventional N-end rule pathway. © 2010 Yang et al.
Share
Citation/Export: |
|
Social Networking: |
|
Details
Item Type: |
Article
|
Status: |
Published |
Creators/Authors: |
Creators | Email | Pitt Username | ORCID  |
---|
Yang, F | | | | Cheng, Y | | | | An, JY | | | | Kwon, YT | yok5@pitt.edu | YOK5 | | Eckardt, S | | | | Leu, NA | | | | Mclaughlin, KJ | | | | Wang, PJ | | | |
|
Contributors: |
Contribution | Contributors Name | Email | Pitt Username | ORCID  |
---|
Editor | Orban, Laszlo | UNSPECIFIED | UNSPECIFIED | UNSPECIFIED |
|
Date: |
3 December 2010 |
Date Type: |
Publication |
Journal or Publication Title: |
PLoS ONE |
Volume: |
5 |
Number: |
11 |
DOI or Unique Handle: |
10.1371/journal.pone.0014017 |
Refereed: |
Yes |
MeSH Headings: |
Animals; Binding Sites--genetics; Blotting, Western; Cysteine--genetics; Female; Immunoprecipitation; Male; Methionine--genetics; Mice; Mice, 129 Strain; Mice, Inbred C57BL; Mice, Knockout; NIH 3T3 Cells; Nuclear Proteins--genetics; Nuclear Proteins--metabolism; Protein Binding; Protein Stability; Signal Transduction; Spermatogenesis; Testis--cytology; Testis--metabolism; Ubiquitin-Protein Ligases--genetics; Ubiquitin-Protein Ligases--metabolism |
Other ID: |
NLM PMC2982839 |
PubMed Central ID: |
PMC2982839 |
PubMed ID: |
21103378 |
Date Deposited: |
22 Aug 2012 21:54 |
Last Modified: |
04 Feb 2019 15:58 |
URI: |
http://d-scholarship.pitt.edu/id/eprint/13576 |
Metrics
Monthly Views for the past 3 years
Plum Analytics
Altmetric.com
Actions (login required)
 |
View Item |