Morris, P and Marinelli, LJ and Jacobs-Sera, D and Hendrix, RW and Hatfull, GF
(2008)
Genomic characterization of mycobacteriophage giles: Evidence for phage acquisition of host DNA by illegitimate recombination.
Journal of Bacteriology, 190 (6).
2172 - 2182.
ISSN 0021-9193
Abstract
A characteristic feature of bacteriophage genomes is that they are architecturally mosaic, with each individual genome representing a unique assemblage of individual exchangeable modules. Plausible mechanisms for generating mosaicism include homologous recombination at shared boundary sequences of module junctions, illegitimate recombination in a non-sequence-directed process, and site-specific recombination. Analysis of the novel mycobacteriophage Giles genome not only extends our current perspective on bacteriophage genetic diversity, with more than 60% of the genes unrelated to other mycobacteriophages, but offers novel insights into how mosaic genomes are created. In one example, the integration/excision cassette is atypically situated within the structural gene operon and could have moved there either by illegitimate recombination or more plausibly via integrase-mediated site-specific recombination. In a second example, a DNA segment has been recently acquired from the host bacterial chromosome by illegitimate recombination, providing further evidence that phage genomic mosaicism is generated by nontargeted recombination processes. Copyright © 2008, American Society for Microbiology. All Rights Reserved.
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Details
| Item Type: |
Article
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| Status: |
Published |
| Creators/Authors: |
|
| Date: |
1 March 2008 |
| Date Type: |
Publication |
| Journal or Publication Title: |
Journal of Bacteriology |
| Volume: |
190 |
| Number: |
6 |
| Page Range: |
2172 - 2182 |
| DOI or Unique Handle: |
10.1128/jb.01657-07 |
| Schools and Programs: |
Dietrich School of Arts and Sciences > Biological Sciences |
| Refereed: |
Yes |
| ISSN: |
0021-9193 |
| MeSH Headings: |
Base Sequence; DNA, Bacterial--genetics; Genome, Viral--genetics; Host-Pathogen Interactions; Microscopy, Electron; Molecular Sequence Data; Mycobacteriophages--genetics; Mycobacteriophages--physiology; Mycobacteriophages--ultrastructure; Mycobacterium smegmatis--genetics; Mycobacterium smegmatis--virology; Operon--genetics; Recombination, Genetic; Sequence Homology, Nucleic Acid |
| Other ID: |
NLM PMC2258872 |
| PubMed Central ID: |
PMC2258872 |
| PubMed ID: |
18178732 |
| Date Deposited: |
03 Dec 2012 22:43 |
| Last Modified: |
28 Sep 2022 13:15 |
| URI: |
http://d-scholarship.pitt.edu/id/eprint/16366 |
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