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Progress in developing polymerized crystalline colloidal array sensors for point-of-care detection of myocardial ischemia

Baca, JT and Finegold, DN and Asher, SA (2008) Progress in developing polymerized crystalline colloidal array sensors for point-of-care detection of myocardial ischemia. Analyst, 133 (3). 385 - 390. ISSN 0003-2654

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Abstract

The difficulty of rapid, definitive diagnosis of myocardial ischemia leads to unnecessary hospital admissions and treatment delays. Previously, decreased metal binding affinity in human serum was investigated as a marker for myocardial ischemia. Polymerized Crystalline Colloidal Array (PCCA) sensors for Ni may be useful in developing a point-of-care test to determine metal binding affinity in plasma and to help rule out myocardial ischemia. PCCA sensors for Ni , with 5-amino-8-hydroxyquinoline as a chelating agent, were tested in aqueous solutions and diluted human plasma. The peak wavelength diffracted by the sensors was monitored by reflectance spectrometry and correlated with Ni concentration. The PCCA sensors show a linear response to aqueous Ni concentrations between 0.2 and 1.0 mmol L , and can detect changes in free Ni concentration of <60 μmol L . The sensors respond at physiologic pH and can be reversibly dehydrated. The PCCA sensors developed here can report on free Ni concentration in the presence of human plasma. These sensors can be used to detect a decrease in the Ni affinity of plasma proteins, which may indicate recent myocardial ischemia. PCCA sensors offer a practical approach to rapid, point-of-care detection of a proposed biochemical signature of myocardial ischemia. © The Royal Society of Chemistry. 2+ 2+ 2+ 2+ -1 2+ -1 2+ 2+


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Baca, JT
Finegold, DN
Asher, SAasher@pitt.eduASHER
Date: 1 January 2008
Date Type: Publication
Journal or Publication Title: Analyst
Volume: 133
Number: 3
Page Range: 385 - 390
DOI or Unique Handle: 10.1039/b712482a
Schools and Programs: Dietrich School of Arts and Sciences > Chemistry
Refereed: Yes
ISSN: 0003-2654
MeSH Headings: Biological Markers--blood; Colloids; Crystallization; Equipment Design; Humans; Hydrogen-Ion Concentration; Myocardial Ischemia--diagnosis; Nickel--blood; Point-of-Care Systems
PubMed ID: 18299754
Date Deposited: 21 Feb 2013 22:36
Last Modified: 30 Mar 2021 11:55
URI: http://d-scholarship.pitt.edu/id/eprint/17336

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