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Exponential-phase glycogen recycling is essential for growth of Mycobacterium smegmatis

Belanger, AE and Hatfull, GF (1999) Exponential-phase glycogen recycling is essential for growth of Mycobacterium smegmatis. Journal of Bacteriology, 181 (21). 6670 - 6678. ISSN 0021-9193

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Abstract

Bacterial glycogen is a polyglucose storage compound that is thought to prolong viability during stationary phase. However, a specific role for glycogen has not been determined. We have characterized SMEG53, a temperature-sensitive mutant of Mycobacterium smegmatis that contains a mutation in glgE, encoding a putative glucanase. This mutation causes exponentially growing SMEG53 cells to stop growing at 42°C in response to high levels of glycogen accumulation. The mutation in glgE is also associated with an altered growth rate and colony morphology at permissive temperatures; the severity of these phenotypes correlates with the amount of glycogen accumulated by the mutant. Suppression of the temperature-sensitive phenotype, via a decrease in glycogen accumulation, is mediated by growth in certain media or multicopy expression of garA. The function of GarA is unknown, but the presence of a forkhead-associated domain suggests that this protein is a member of a serine-threonine kinase signal transduction pathway. Our results suggest that in M. smegmatis glycogen is continuously synthesized and then degraded by GlgE throughout exponential growth. In turn, this constant recycling of glycogen controls the downstream availability of carbon and energy. Thus, in addition to its conventional storage role, glycogen may also serve as a carbon capacitor for glycolysis during the exponential growth of M. smegmatis.

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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Belanger, AE
Hatfull, GFgfh@pitt.eduGFH
Date: 1 January 1999
Date Type: Publication
Journal or Publication Title: Journal of Bacteriology
Volume: 181
Number: 21
Page Range: 6670 - 6678
DOI or Unique Handle: 10.1128/jb.181.21.6670-6678.1999
Schools and Programs: Dietrich School of Arts and Sciences > Biological Sciences
Refereed: Yes
ISSN: 0021-9193
MeSH Headings: Amino Acid Sequence; Bacterial Proteins--genetics; Bacterial Proteins--metabolism; Gene Expression Regulation, Bacterial; Genetic Complementation Test; Glycogen--metabolism; Glycoside Hydrolases--chemistry; Glycoside Hydrolases--genetics; Glycoside Hydrolases--metabolism; Molecular Sequence Data; Mutagenesis; Mycobacterium smegmatis--drug effects; Mycobacterium smegmatis--enzymology; Mycobacterium smegmatis--growth & development; Nitrosoguanidines--pharmacology; Polymerase Chain Reaction--methods; Protein-Serine-Threonine Kinases--genetics; Protein-Serine-Threonine Kinases--metabolism; Sequence Analysis, DNA; Temperature
Other ID: NLM PMC94131
PubMed Central ID: PMC94131
PubMed ID: 10542168
Date Deposited: 01 Mar 2013 18:47
Last Modified: 28 Sep 2022 13:17
URI: http://d-scholarship.pitt.edu/id/eprint/17602

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