Cooper, GM and Miller, ED and Decesare, GE and Usas, A and Lensie, EL and Bykowski, MR and Huard, J and Weiss, LE and Losee, JE and Campbell, PG
(2010)
Inkjet-based biopatterning of bone morphogenetic protein-2 to spatially control calvarial bone formation.
Tissue Engineering - Part A, 16 (5).
1749 - 1759.
ISSN 1937-3341
Abstract
The purpose of this study was to demonstrate spatial control of osteoblast differentiation in vitro and bone formation in vivo using inkjet bioprinting technology and to create three-dimensional persistent bio-ink patterns of bone morphogenetic protein-2 (BMP-2) and its modifiers immobilized within microporous scaffolds. Semicircular patterns of BMP-2 were printed within circular DermaMatrix™ human allograft scaffold constructs. The contralateral halves of the constructs were unprinted or printed with BMP-2 modifiers, including the BMP-2 inhibitor, noggin. Printed bio-ink pattern retention was validated using fluorescent or 125I-labeled bio-inks. Mouse C2C12 progenitor cells cultured on patterned constructs differentiated in a dose-dependent fashion toward an osteoblastic fate in register to BMP-2 patterns. The fidelity of spatial restriction of osteoblastic differentiation at the boundary between neighboring BMP-2 and noggin patterns improved in comparison with patterns without noggin. Acellular DermaMatrix constructs similarly patterned with BMP-2 and noggin were then implanted into a mouse calvarial defect model. Patterns of bone formation in vivo were comparable with patterned responses of osteoblastic differentiation in vitro. These results demonstrate that three-dimensional biopatterning of a growth factor and growth factor modifier within a construct can direct cell differentiation in vitro and tissue formation in vivo in register to printed patterns. © 2010 Mary Ann Liebert, Inc.
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Item Type: |
Article
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Status: |
Published |
Creators/Authors: |
Creators | Email | Pitt Username | ORCID  |
---|
Cooper, GM | gmc8@pitt.edu | GMC8 | | Miller, ED | | | | Decesare, GE | | | | Usas, A | | | | Lensie, EL | | | | Bykowski, MR | | | | Huard, J | | | | Weiss, LE | | | | Losee, JE | joseph.losee@pitt.edu | JEL35 | | Campbell, PG | | | |
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Centers: |
Other Centers, Institutes, Offices, or Units > Stem Cell Research Center |
Date: |
1 May 2010 |
Date Type: |
Publication |
Journal or Publication Title: |
Tissue Engineering - Part A |
Volume: |
16 |
Number: |
5 |
Page Range: |
1749 - 1759 |
DOI or Unique Handle: |
10.1089/ten.tea.2009.0650 |
Schools and Programs: |
School of Medicine > Surgery Swanson School of Engineering > Bioengineering |
Refereed: |
Yes |
ISSN: |
1937-3341 |
MeSH Headings: |
Animals; Bone Morphogenetic Protein 2--pharmacology; Cell Differentiation--drug effects; Humans; Male; Mice; Mice, Inbred C57BL; Microscopy, Electron, Scanning; Osteogenesis--drug effects; Skull--drug effects; Skull--growth & development; Tissue Engineering--instrumentation; Tissue Engineering--methods; Tissue Scaffolds--chemistry |
Other ID: |
NLM PMC2952127 |
PubMed Central ID: |
PMC2952127 |
PubMed ID: |
20028232 |
Date Deposited: |
04 Apr 2014 16:27 |
Last Modified: |
07 Jan 2023 11:56 |
URI: |
http://d-scholarship.pitt.edu/id/eprint/20931 |
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