Epperly, MW and Goff, JP and Li, S and Gao, X and Wipf, P and Dixon, T and Wang, H and Franicola, D and Shen, H and Rwigema, JCM and Kagan, V and Bernard, M and Greenberger, JS
(2010)
Intraesophageal administration of GS-nitroxide (JP4-039) protects against ionizing irradiation-induced esophagitis.
In Vivo, 24 (6).
811 - 819.
ISSN 0258-851X
Abstract
Background/Aim: This study evaluated esophageal radioprotection by the Gramicidin S (GS) derived-nitroxide, JP4-039, a mitochondrial targeting peptide-isostere covalently-linked to 4-amino-Tempo, delivered in a novel swallowed oil-based (F15) formulation. Materials and Methods: C57BL/6HNsd female mice received intraesophageal F15 formulation containing JP4-039 (4 mg/ml in 100 μl volumes) 10 minutes before 28 or 29 Gy upper body irradiation compared to MnSOD-PL (100 μl containing 100 μg plasmid) 24 hours prior to irradiation. Subgroups received 1×107 C57BL/6HNsd, GFP + male bone marrow cells intravenously 5 days after irradiation. Results: JP4-039/F15 or MnSOD-PL increased survival compared to irradiated controls (p<0.0001 for either). Marrow injection further increased survival (p=0.0462 and 0.0351, respectively). Esophagi removed at 1, 3, 7, 14, 24, or 60 days showed bone marrow-derived cells in the esophagi. Conclusion: Intraesophageal GS-nitroxide radioprotection is mediated primarily through recovery of endogenous esophageal progenitor cells.
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Item Type: |
Article
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Status: |
Published |
Creators/Authors: |
Creators | Email | Pitt Username | ORCID  |
---|
Epperly, MW | | | | Goff, JP | | | | Li, S | sol4@pitt.edu | SOL4 | | Gao, X | | | | Wipf, P | pwipf@pitt.edu | PWIPF | | Dixon, T | | | | Wang, H | how8@pitt.edu | HOW8 | 0000-0003-0477-2908 | Franicola, D | | | | Shen, H | hos1@pitt.edu | HOS1 | | Rwigema, JCM | | | | Kagan, V | kagan@pitt.edu | KAGAN | | Bernard, M | | | | Greenberger, JS | joelg@pitt.edu | JOELG | |
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Date: |
1 January 2010 |
Date Type: |
Publication |
Journal or Publication Title: |
In Vivo |
Volume: |
24 |
Number: |
6 |
Page Range: |
811 - 819 |
Schools and Programs: |
Dietrich School of Arts and Sciences > Chemistry |
Refereed: |
Yes |
ISSN: |
0258-851X |
MeSH Headings: |
Animals; Bone Marrow Transplantation; Cytoprotection; Drug Delivery Systems; Esophagitis--pathology; Esophagitis--prevention & control; Esophagitis--therapy; Esophagus--drug effects; Esophagus--pathology; Esophagus--radiation effects; Female; Liposomes--therapeutic use; Male; Mice; Mice, Inbred C57BL; Nitrogen Oxides--administration & dosage; Nitrogen Oxides--pharmacokinetics; Nitrogen Oxides--pharmacology; Radiation Injuries, Experimental--pathology; Radiation Injuries, Experimental--prevention & control; Radiation Injuries, Experimental--therapy |
Other ID: |
NLM NIHMS426447, NLM PMC3521523 |
PubMed Central ID: |
PMC3521523 |
PubMed ID: |
21164038 |
Date Deposited: |
31 Jul 2014 20:45 |
Last Modified: |
26 Sep 2022 16:23 |
URI: |
http://d-scholarship.pitt.edu/id/eprint/22340 |
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