Pope-Varsalona, Hannah C
(2014)
The role of polymerase η in protecting against genome instability and telomere defects caused by the generation of environmentally relevant DNA lesions.
Doctoral Dissertation, University of Pittsburgh.
(Unpublished)
Abstract
Telomeres, the protective caps at chromosome ends, shorten with age in most human cell types, but may be shortened prematurely by DNA damaging agents. Defective telomeres contribute to aging-related diseases and may give rise to genomic alterations implicated in carcinogenesis. Translesion DNA synthesis is a critical cellular mechanism that ensures progression of DNA replication forks, most notably, in the face of bulky DNA lesions. Numerous environmental exposures generate bulky lesions, such as ultraviolet (UV) light and hexavalent chromium (Cr(VI)). Translesion synthesis polymerase η’s (polη) role in protecting against UV-induced lesions in the genome has been extensively documented, but its role at telomeres is unknown. Additionally, UV-induced lesions have been shown to form at telomeres. Chronic inhalation of Cr(VI) induces respiratory diseases associated with aging and telomere dysfunction, including pulmonary fibrosis and cancers, and our previous work established that Cr(VI) causes telomere damage. However, the mechanism(s) by which environmental genotoxicants promote telomere loss and defects is unknown. We investigated roles for polη in preserving telomeres following acute physical UVC exposure and chronic chemical Cr(VI) exposure. Similar to its role in protecting against UV-induced DNA damage, we report that polη protects against cytotoxicity and DNA replication stress caused by Cr(VI). Our study supports a novel role for translesion DNA synthesis in preserving telomeres after UVC and Cr(VI) exposure and genotoxic stress. We uncover a mechanism by which environmental genotoxicants alter telomere integrity, and a fundamental cellular pathway that preserves telomere function in the face of genotoxic replication stress. Telomere alterations and dysfunction have been shown to impact human health. This research is significant and relevant to public health because knowledge gained will be useful for designing intervention therapies that preserve telomeres in human populations following exposure to environmental genotoxicants. The hope is that preventative measures will inhibit or delay diseases and pathologies related to telomere defects.
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Details
| Item Type: |
University of Pittsburgh ETD
|
| Status: |
Unpublished |
| Creators/Authors: |
| Creators | Email | Pitt Username | ORCID  |
|---|
| Pope-Varsalona, Hannah C | hcp8@pitt.edu | HCP8 | |
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| ETD Committee: |
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| Date: |
29 September 2014 |
| Date Type: |
Publication |
| Defense Date: |
28 July 2014 |
| Approval Date: |
29 September 2014 |
| Submission Date: |
21 July 2014 |
| Access Restriction: |
2 year -- Restrict access to University of Pittsburgh for a period of 2 years. |
| Number of Pages: |
142 |
| Institution: |
University of Pittsburgh |
| Schools and Programs: |
School of Public Health > Environmental and Occupational Health |
| Degree: |
PhD - Doctor of Philosophy |
| Thesis Type: |
Doctoral Dissertation |
| Refereed: |
Yes |
| Uncontrolled Keywords: |
telomeres, translesion synthesis, polymerase η, UV, chromium |
| Date Deposited: |
29 Sep 2014 21:07 |
| Last Modified: |
19 Jul 2024 18:47 |
| URI: |
http://d-scholarship.pitt.edu/id/eprint/22443 |
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