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Characterization of the dnaG locus in Mycobacterium smegmatis reveals linkage of DNA replication and cell division

Klann, AG and Belanger, AE and Abanes-de Mello, A and Lee, JY and Hatfull, GF (1998) Characterization of the dnaG locus in Mycobacterium smegmatis reveals linkage of DNA replication and cell division. Journal of Bacteriology, 180 (1). 65 - 72. ISSN 0021-9193

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Abstract

We have isolated a UV-induced temperature-sensitive mutant of Mycobacterium smegmatis that fails to grow at 42°C and exhibits a filamentous phenotype following incubation at the nonpermissive temperature, reminiscent of a defect in cell division. Complementation of this mutant with an M. smegmatis genomic library and subsequent subcloning reveal that the defect lies within the M. smegmatis dnaG gene encoding DNA primase. Sequence analysis of the mutant dnaG allele reveals a substitution of proline for alanine at position 496. Thus, dnaG is an essential gene in M. smegmatis, and DNA replication and cell division are coupled processes in this species. Characterization of the sequences flanking the M. smegmatis dnaG gene shows that it is not part of the highly conserved macromolecular synthesis operon present in other eubacterial species but is part of an operon with a dgt gene encoding dGTPase. The organization of this operon is conserved in Mycobacterium tuberculosis and Mycobacterium leprae, suggesting that regulation of DNA replication, transcription, and translation may be coordinated differently in the mycobacteria than in other bacteria.

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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Klann, AG
Belanger, AE
Abanes-de Mello, A
Lee, JY
Hatfull, GFgfh@pitt.eduGFH
Date: 1 January 1998
Date Type: Publication
Journal or Publication Title: Journal of Bacteriology
Volume: 180
Number: 1
Page Range: 65 - 72
DOI or Unique Handle: 10.1128/jb.180.1.65-72.1998
Schools and Programs: Dietrich School of Arts and Sciences > Biological Sciences
Refereed: Yes
ISSN: 0021-9193
MeSH Headings: Alleles; Amino Acid Sequence; Cell Division--genetics; DNA Mutational Analysis; DNA Primase--genetics; DNA Replication--genetics; Genes, Bacterial--genetics; Genetic Complementation Test; Genetic Linkage; Molecular Sequence Data; Mutation; Mycobacterium--cytology; Mycobacterium--genetics; Open Reading Frames--genetics; Operon--genetics; Restriction Mapping; Sequence Homology, Amino Acid; Temperature
Other ID: NLM PMC106850
PubMed Central ID: PMC106850
PubMed ID: 9422594
Date Deposited: 17 Nov 2014 19:59
Last Modified: 28 Sep 2022 13:22
URI: http://d-scholarship.pitt.edu/id/eprint/23481

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