Robinson, AC and Kenan, DJ and Hatfull, GF and Sullivan, NF and Spiegelberg, R and Donachie, WD
(1984)
DNA sequence and transcriptional organization of essential cell division genes ftsQ and ftsA of Escherichia coli: Evidence for overlapping transcriptional units.
Journal of Bacteriology, 160 (2).
546 - 555.
ISSN 0021-9193
Abstract
The DNA sequence of a cloned segment of the Escherichia coli chromosome containing ftsQ, ftsA, and part of the ftsZ gene was determined and interpreted for genetic complementation and promoter fusion data for the region. The contiguous gene ftsQ, ftsA, and ftsZ were transcribed in the same direction (clockwise on the genetic map) and each had at least one associated promoter which allowed it to transcribed independently of neighboring genes. ftsA and ftsZ possessed promoters within the coding sequences of the juxtaposed upstream structural genes, and a promoter element for ftsA was surrounded by a region of twofold symmetry which corresponded closely to a symmetrical element in the region of a putative ftsZ promoter. The structural gene of ftsQ consisted of 838 nucleotides, encoding a 276-residue amino acid polypeptide of molecular weight 31,400; the structural gene of ftsA consisted of 1,260 nucleotides, encoding a 420-residue amino acid polypeptide of molecular weight 45,400. The observation that the termination codon of ftsQ overlaps with a potential initiation codon for ftsA suggested that these two genes may be translationally coupled when transcription is initiated upstream of the ftsQ coding sequence.
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Item Type: |
Article
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Status: |
Published |
Creators/Authors: |
Creators | Email | Pitt Username | ORCID |
---|
Robinson, AC | | | | Kenan, DJ | | | | Hatfull, GF | gfh@pitt.edu | GFH | | Sullivan, NF | | | | Spiegelberg, R | | | | Donachie, WD | | | |
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Date: |
1 December 1984 |
Date Type: |
Publication |
Journal or Publication Title: |
Journal of Bacteriology |
Volume: |
160 |
Number: |
2 |
Page Range: |
546 - 555 |
Schools and Programs: |
Dietrich School of Arts and Sciences > Biological Sciences |
Refereed: |
Yes |
ISSN: |
0021-9193 |
MeSH Headings: |
Amino Acid Sequence; Base Sequence; Cell Division; Cloning, Molecular; DNA Restriction Enzymes; DNA, Bacterial--genetics; Escherichia coli--cytology; Escherichia coli--genetics; Genes; Genes, Regulator; Plasmids; Promoter Regions, Genetic; Transcription, Genetic |
Other ID: |
NLM PMC214769 |
PubMed Central ID: |
PMC214769 |
PubMed ID: |
6094474 |
Date Deposited: |
19 Nov 2014 20:20 |
Last Modified: |
02 Feb 2019 15:57 |
URI: |
http://d-scholarship.pitt.edu/id/eprint/23548 |
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