Comparative genomics of Cluster O mycobacteriophages

Cresawn, SG and Pope, WH and Jacobs-Sera, D and Bowman, CA and Russell, DA and Dedrick, RM and Adair, T and Anders, KR and Ball, S and Bollivar, D and Breitenberger, C and Burnett, SH and Butela, K and Byrnes, D and Carzo, S and Cornely, KA and Cross, T and Daniels, RL and Dunbar, D and Findley, AM and Gissendanner, CR and Golebiewska, UP and Hartzog, GA and Hatherill, JR and Hughes, LE and Jalloh, CS and De Los Santos, C and Ekanem, K and Khambule, SL and King, RA and King-Smith, C and Klyczek, K and Krukonis, GP and Laing, C and Lapin, JS and Lopez, AJ and Mkhwanazi, SM and Molloy, SD and Moran, D and Munsamy, V and Pacey, E and Plymale, R and Poxleitner, M and Reyna, N and Schildbach, JF and Stukey, J and Taylor, SE and Ware, VC and Wellmann, AL and Westholm, D and Wodarski, D and Zajko, M and Zikalala, TS and Hendrix, RW and Hatfull, GF (2015) Comparative genomics of Cluster O mycobacteriophages. PLoS ONE, 10 (3).

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Abstract

Mycobacteriophages - viruses of mycobacterial hosts - are genetically diverse but morphologically are all classified in the Caudovirales with double-stranded DNA and tails. We describe here a group of five closely related mycobacteriophages - Corndog, Catdawg, Dylan, Firecracker, and YungJamal - designated as Cluster O with long flexible tails but with unusual prolate capsids. Proteomic analysis of phage Corndog particles, Catdawg particles, and Corndog-infected cells confirms expression of half of the predicted gene products and indicates a non-canonical mechanism for translation of the Corndog tape measure protein. Bioinformatic analysis identifies 8-9 strongly predicted SigA promoters and all five Cluster O genomes contain more than 30 copies of a 17 bp repeat sequence with dyad symmetry located throughout the genomes. Comparison of the Cluster O phages provides insights into phage genome evolution including the processes of gene flux by horizontal genetic exchange.

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Details

Item Type:	Article
Status:	Published
Creators/Authors:	Creators Email Pitt Username ORCID Cresawn, SG Pope, WH Jacobs-Sera, D djs@pitt.edu DJS 0000-0001-7838-3257 Bowman, CA Russell, DA dar78@pitt.edu DAR78 0000-0001-6894-2748 Dedrick, RM dedrick@pitt.edu DEDRICK Adair, T Anders, KR Ball, S Bollivar, D Breitenberger, C Burnett, SH Butela, K KAB340@pitt.edu KAB340 Byrnes, D Carzo, S Cornely, KA Cross, T Daniels, RL Dunbar, D Findley, AM Gissendanner, CR Golebiewska, UP Hartzog, GA Hatherill, JR Hughes, LE Jalloh, CS De Los Santos, C Ekanem, K Khambule, SL King, RA King-Smith, C Klyczek, K Krukonis, GP Laing, C Lapin, JS Lopez, AJ Mkhwanazi, SM Molloy, SD Moran, D Munsamy, V Pacey, E Plymale, R Poxleitner, M Reyna, N Schildbach, JF Stukey, J Taylor, SE Ware, VC Wellmann, AL Westholm, D Wodarski, D Zajko, M Zikalala, TS Hendrix, RW rhx@pitt.edu RHX Hatfull, GF gfh@pitt.edu GFH
Contributors:	Contribution Contributors Name Email Pitt Username ORCID Editor van Raaij, Mark J. UNSPECIFIED UNSPECIFIED UNSPECIFIED
Date:	5 March 2015
Date Type:	Publication
Journal or Publication Title:	PLoS ONE
Volume:	10
Number:	3
DOI or Unique Handle:	10.1371/journal.pone.0118725
Schools and Programs:	Dietrich School of Arts and Sciences > Biological Sciences
Refereed:	Yes
Article Type:	Review
PubMed ID:	25742016
Date Deposited:	12 May 2015 18:06
Last Modified:	30 Mar 2021 10:55
URI:	http://d-scholarship.pitt.edu/id/eprint/24099

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