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The gamma delta resolvase induces an unusual DNA structure at the recombinational crossover point.

Hatfull, GF and Noble, SM and Grindley, ND (1987) The gamma delta resolvase induces an unusual DNA structure at the recombinational crossover point. Cell, 49 (1). 103 - 110. ISSN 0092-8674

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gamma delta resolvase, a transposon-encoded protein active in site-specific recombination, induces a structural change in the DNA at the recombinational crossover point that results in enhanced intercalation of the foot-printing reagent MPE X Fe(II). The structural change correlates with the formation of a bend in the DNA: a mutant resolvase that binds to the crossover site but induces little or no bend does not promote intercalation. The properties of the mutant protein suggest that the induced structural change, which we propose is a localized kink, is required for recombination.


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Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
Hatfull, GFgfh@pitt.eduGFH
Noble, SM
Grindley, ND
Date: 10 April 1987
Date Type: Publication
Journal or Publication Title: Cell
Volume: 49
Number: 1
Page Range: 103 - 110
DOI or Unique Handle: 10.1016/0092-8674(87)90760-4
Schools and Programs: Dietrich School of Arts and Sciences > Biological Sciences
Refereed: Yes
Uncontrolled Keywords: Crossing Over, Genetic, DNA, DNA Transposable Elements, Genetic Complementation Test, Models, Molecular, Mutation, Nucleic Acid Conformation, Nucleotidyltransferases, Plasmids, Protein Binding, Recombination, Genetic, Transposases
ISSN: 0092-8674
Funders: NIGMS NIH HHS (GM28470)
MeSH Headings: Crossing Over, Genetic; DNA--genetics; DNA Transposable Elements; Genetic Complementation Test; Models, Molecular; Mutation; Nucleic Acid Conformation; Nucleotidyltransferases--metabolism; Plasmids; Protein Binding; Recombination, Genetic; Transposases
PubMed ID: 3030563
Date Deposited: 23 Nov 2015 15:03
Last Modified: 14 Jul 2019 02:55


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