Weber, Megan
(2016)
EXAMINING THE RELATIONSHIP BETWEEN THE GENOTYPE AND REDEFINED PHENOTYPE OF DENTAL CARIES.
Doctoral Dissertation, University of Pittsburgh.
(Unpublished)
Abstract
Dental caries is a multifactorial infectious disease and is a major public health problem estimated to affect 60 to 90 percent of school children as well as a vast number of adults. At this time, the best predictor of dental caries for a patient is whether or not they have had caries in the past. We have eight hundred seventy-six whole saliva samples collected from Norwegian patients from one of five primary care dental centers in Rogaland County. The University of Pittsburgh IRB under protocol #12110620 approved these study samples. dmft/DMFT scores were collected at ages 5, 12, 14, 16, 17, and 18 according to World Health Organization (WHO) Protocols. DNA was extracted from whole saliva using Oragene kits and an established protocol. Our objective was to use the dmft/DMFT scores over time to redefine the phenotype of caries and look for patterns of the disease in order to assist in the identification of individuals at risk for caries and aid in the development of new strategies of prevention. We aimed to categorize the patients according to characteristics such as more or less acute increases in their dmft/DMFT scores. We also aimed to compare our newly formed definitions with definitions based on dmft/DMFT cutoffs we have been using in our studies. We generated genotyping data using Taqman chemistry in SNPs involved in processes such as enamel formation and salivary contributions and test for association with caries defined using longitudinal caries experience scores. We compared dmft/DMFT data to diet data and dental erosion scores to determine the role of diet within our population and determine if dental caries and dental erosion scores are associated. Funding for this study came from NIH/NIDCR R01-DE18914 and graduate support from the University of Pittsburgh School of Dental Medicine.
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Details
| Item Type: |
University of Pittsburgh ETD
|
| Status: |
Unpublished |
| Creators/Authors: |
|
| ETD Committee: |
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| Date: |
12 September 2016 |
| Date Type: |
Publication |
| Defense Date: |
12 August 2016 |
| Approval Date: |
12 September 2016 |
| Submission Date: |
11 September 2016 |
| Access Restriction: |
No restriction; Release the ETD for access worldwide immediately. |
| Number of Pages: |
211 |
| Institution: |
University of Pittsburgh |
| Schools and Programs: |
School of Dental Medicine > Dental Science |
| Degree: |
PhD - Doctor of Philosophy |
| Thesis Type: |
Doctoral Dissertation |
| Refereed: |
Yes |
| Uncontrolled Keywords: |
Dental Caries, Dental Erosion, Genetics, Genotyping |
| Date Deposited: |
12 Sep 2016 14:58 |
| Last Modified: |
15 Nov 2016 14:36 |
| URI: |
http://d-scholarship.pitt.edu/id/eprint/29516 |
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