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Identifier mapping performance for integrating transcriptomics and proteomics experimental results

Day, RS and McDade, KK and Chandran, UR and Lisovich, A and Conrads, TP and Hood, BL and Kolli, VSK and Kirchner, D and Litzi, T and Maxwell, GL (2011) Identifier mapping performance for integrating transcriptomics and proteomics experimental results. BMC Bioinformatics, 12.

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Abstract

Background: Studies integrating transcriptomic data with proteomic data can illuminate the proteome more clearly than either separately. Integromic studies can deepen understanding of the dynamic complex regulatory relationship between the transcriptome and the proteome. Integrating these data dictates a reliable mapping between the identifier nomenclature resultant from the two high-throughput platforms. However, this kind of analysis is well known to be hampered by lack of standardization of identifier nomenclature among proteins, genes, and microarray probe sets. Therefore data integration may also play a role in critiquing the fallible gene identifications that both platforms emit.Results: We compared three freely available internet-based identifier mapping resources for mapping UniProt accessions (ACCs) to Affymetrix probesets identifications (IDs): DAVID, EnVision, and NetAffx. Liquid chromatography-tandem mass spectrometry analyses of 91 endometrial cancer and 7 noncancer samples generated 11,879 distinct ACCs. For each ACC, we compared the retrieval sets of probeset IDs from each mapping resource. We confirmed a high level of discrepancy among the mapping resources. On the same samples, mRNA expression was available. Therefore, to evaluate the quality of each ACC-to-probeset match, we calculated proteome-transcriptome correlations, and compared the resources presuming that better mapping of identifiers should generate a higher proportion of mapped pairs with strong inter-platform correlations. A mixture model for the correlations fitted well and supported regression analysis, providing a window into the performance of the mapping resources. The resources have added and dropped matches over two years, but their overall performance has not changed.Conclusions: The methods presented here serve to achieve concrete context-specific insight, to support well-informed decisions in choosing an ID mapping strategy for "omic" data merging. © 2011 Day et al; licensee BioMed Central Ltd.

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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Day, RSday01@pitt.eduDAY01
McDade, KKkkm5@pitt.eduKKM5
Chandran, URchandran@pitt.eduCHANDRAN
Lisovich, A
Conrads, TP
Hood, BL
Kolli, VSK
Kirchner, D
Litzi, T
Maxwell, GL
Date: 27 May 2011
Date Type: Publication
Journal or Publication Title: BMC Bioinformatics
Volume: 12
DOI or Unique Handle: 10.1186/1471-2105-12-213
Schools and Programs: School of Public Health > Biostatistics
School of Medicine > Biomedical Informatics
Refereed: Yes
Date Deposited: 27 Oct 2016 19:24
Last Modified: 02 Feb 2019 16:56
URI: http://d-scholarship.pitt.edu/id/eprint/30055

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