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Association analysis of PON2 genetic variants with serum paraoxonase activity and systemic lupus erythematosus

Dasgupta, S and Demirci, FY and Dressen, AS and Kao, AH and Rhew, EY and Ramsey-Goldman, R and Manzi, S and Kammerer, CM and Kamboh, MI (2011) Association analysis of PON2 genetic variants with serum paraoxonase activity and systemic lupus erythematosus. BMC Medical Genetics, 12.

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Abstract

Background: Low serum paraoxonase (PON) activity is associated with the risk of coronary artery disease, diabetes and systemic lupus erythematosus (SLE). Our prior studies have shown that the PON1/rs662 (p.Gln192Arg), PON1/rs854560 (p.Leu55Met), PON3/rs17884563 and PON3/rs740264 SNPs (single nucleotide polymorphisms) significantly affect serum PON activity. Since PON1, PON2 and PON3 share high degree of structural and functional properties, in this study, we examined the role of PON2 genetic variation on serum PON activity, risk of SLE and SLE-related clinical manifestations in a Caucasian case-control sample.Methods: PON2 SNPs were selected from HapMap and SeattleSNPs databases by including at least one tagSNP from each bin defined in these resources. A total of nineteen PON2 SNPs were successfully genotyped in 411 SLE cases and 511 healthy controls using pyrosequencing, restriction fragment length polymorphism (RFLP) or TaqMan allelic discrimination methods.Results: Our pair-wise linkage disequilibrium (LD) analysis, using an r cutoff of 0.7, identified 14 PON2 tagSNPs that captured all 19 PON2 variants in our sample, 12 of which were not in high LD with known PON1 and PON3 SNP modifiers of PON activity. Stepwise regression analysis of PON activity, including the known modifiers, identified five PON2 SNPs [rs6954345 (p.Ser311Cys), rs13306702, rs987539, rs11982486, and rs4729189; P = 0.005 to 2.1 × 10 ] that were significantly associated with PON activity. We found no association of PON2 SNPs with SLE risk but modest associations were observed with lupus nephritis (rs11981433, rs17876205, rs17876183) and immunologic disorder (rs11981433) in SLE patients (P = 0.013 to 0.042).Conclusions: Our data indicate that PON2 genetic variants significantly affect variation in serum PON activity and have modest effects on risk of lupus nephritis and SLE-related immunologic disorder. © 2011 Dasgupta et al; licensee BioMed Central Ltd. 2 -6


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Dasgupta, S
Demirci, FYfyd1@pitt.eduFYD1
Dressen, AS
Kao, AH
Rhew, EY
Ramsey-Goldman, R
Manzi, S
Kammerer, CMcmk3@pitt.eduCMK3
Kamboh, MIkamboh@pitt.eduKAMBOH
Date: 11 January 2011
Date Type: Publication
Journal or Publication Title: BMC Medical Genetics
Volume: 12
DOI or Unique Handle: 10.1186/1471-2350-12-7
Schools and Programs: Graduate School of Public Health > Human Genetics
Refereed: Yes
Date Deposited: 16 Nov 2016 18:48
Last Modified: 30 Mar 2021 11:55
URI: http://d-scholarship.pitt.edu/id/eprint/30196

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