Van Oss, S. Branden
(2017)
Insights into the role of the Paf1 complex in promoting H2B monoubiquitylation.
Doctoral Dissertation, University of Pittsburgh.
(Unpublished)
Abstract
The polymerase-associated factor 1 complex (Paf1C) regulates RNA polymerase II transcription and is required for the monoubiquitylation of histone H2B (H2Bub), a conserved epigenetic modification with broad implications for the regulation of gene expression and human health. This dissertation explores the molecular mechanisms by which Paf1C acts to promote H2Bub. Paf1C regulation of H2Bub is governed largely by the histone modification domain (HMD) of
the Rtf1 subunit, which promotes H2Bub even in the absence of all Paf1C subunits and interacts directly with the enzyme that catalyzes H2Bub. The role of the HMD in stimulating H2Bub is direct, and at least partially independent of transcription. My work is part of a collaborative effort to investigate the activity of the HMD through structural, genetic, bioinformatic, and
biochemical approaches. As the influence of H2Bub on human health continues to become more clear, interest in this modification will likely continue to expand.
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Details
| Item Type: |
University of Pittsburgh ETD
|
| Status: |
Unpublished |
| Creators/Authors: |
| Creators | Email | Pitt Username | ORCID  |
|---|
| Van Oss, S. Branden | | | |
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| ETD Committee: |
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| Date: |
28 September 2017 |
| Date Type: |
Publication |
| Defense Date: |
2 August 2017 |
| Approval Date: |
28 September 2017 |
| Submission Date: |
10 August 2017 |
| Access Restriction: |
3 year -- Restrict access to University of Pittsburgh for a period of 3 years. |
| Number of Pages: |
162 |
| Institution: |
University of Pittsburgh |
| Schools and Programs: |
Dietrich School of Arts and Sciences > Biological Sciences |
| Degree: |
PhD - Doctor of Philosophy |
| Thesis Type: |
Doctoral Dissertation |
| Refereed: |
Yes |
| Uncontrolled Keywords: |
chromatin, Paf1 complex, histone modification domain, H2B ubiquitylation, histone modifications, Rtf1 |
| Date Deposited: |
29 Sep 2017 00:59 |
| Last Modified: |
28 Sep 2020 05:15 |
| URI: |
http://d-scholarship.pitt.edu/id/eprint/33066 |
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