Krawchuk, Michael
(2019)
Optogenetic assessment of VIP, PV, SOM, and NOS inhibitory neuron activity and cerebral blood flow regulation in mouse somato-sensory cortex.
Undergraduate Thesis, University of Pittsburgh.
(Unpublished)
Abstract
The impact of different neuronal populations on local cerebral blood flow (CBF) regulation is not well known and insight into these relationships could enhance the interpretation of brain function and dysfunction from brain imaging data. We investigated the role of sub-types of inhibitory neurons on the regulation of CBF using optogenetics, laser Doppler flowmetry and different transgenic mouse models (parvalbumin (PV), vasoactive intestinal peptide (VIP), somatostatin (SOM) and nitric oxide synthase (NOS)). Whisker stimulation was used to verify that typical CBF responses were obtained in all mice (average increase of 14%). Photo-stimulation of SOM-cre and NOS-cre mice produced significant increases in CBF that were similar to whisker responses. In NOS-cre mice, CBF responses scaled with the photo-stimulus pulse duration and frequency. In SOM-cre mice, CBF increases were followed by decreases. In VIP-cre mice, photo-stimulation did not consistently produce significant changes in CBF, while slower increases in CBF that peaked 14-18 seconds after stimulation onset were observed in PV-cre mice. Control experiments performed in non-expressing regions showed no changes in CBF. These findings suggest that dysfunction in NOS or SOM expressing neurons can have a significant impact on CBF responses that could be detected by brain imaging methods like fMRI.
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Details
| Item Type: |
University of Pittsburgh ETD
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| Status: |
Unpublished |
| Creators/Authors: |
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| ETD Committee: |
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| Date: |
22 April 2019 |
| Date Type: |
Publication |
| Defense Date: |
1 April 2019 |
| Approval Date: |
22 April 2019 |
| Submission Date: |
16 April 2019 |
| Access Restriction: |
No restriction; Release the ETD for access worldwide immediately. |
| Number of Pages: |
44 |
| Institution: |
University of Pittsburgh |
| Schools and Programs: |
David C. Frederick Honors College
Dietrich School of Arts and Sciences > Neuroscience |
| Degree: |
BPhil - Bachelor of Philosophy |
| Thesis Type: |
Undergraduate Thesis |
| Refereed: |
Yes |
| Uncontrolled Keywords: |
Blood flow, fMRI, Imaging, Inhibitory, Optogenetics |
| Date Deposited: |
22 Apr 2019 16:23 |
| Last Modified: |
22 Apr 2019 16:23 |
| URI: |
http://d-scholarship.pitt.edu/id/eprint/36512 |
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