David, Sherin
(2020)
Stem Cell and Tissue-Based Therapies for Male Infertility.
Doctoral Dissertation, University of Pittsburgh.
(Unpublished)
Abstract
Infertility due to exposure to chemotherapy and radiation has a detrimental effect of the patient’s quality of life. A number of spermatogonial stem cell (SSC)-based methods are currently being developed for prepubertal male patients that cannot produce sperm for cryopreservation at the time of treatment. Spermatogonial stem cell culture and transplantation, and testicular tissue xenografting are technologies that have been proven to be successful in a number of mammalian species. Efforts to apply these technologies to human SSCs are currently underway. This dissertation describes work carried out to optimize methods for culturing human SSCs in vitro and to promote survival and maturation of cryopreserved and thawed prepubertal primate testicular xenografts in mice. We developed a multiparametric flow cytometry-based method to quantify human SSCs and used it to optimize the substrate and medium for culturing human SSCs. We also showed that human chorionic gonadotropin exposure promotes growth of both human and Rhesus xenografts and complete maturation of Rhesus xenografts. Additional studies need to be carried out to further develop both SSC culture and human xenografting technologies for application in the clinic for fertility preservation.
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Details
| Item Type: |
University of Pittsburgh ETD
|
| Status: |
Unpublished |
| Creators/Authors: |
|
| ETD Committee: |
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| Date: |
4 May 2020 |
| Date Type: |
Publication |
| Defense Date: |
5 February 2020 |
| Approval Date: |
4 May 2020 |
| Submission Date: |
23 April 2020 |
| Access Restriction: |
1 year -- Restrict access to University of Pittsburgh for a period of 1 year. |
| Number of Pages: |
157 |
| Institution: |
University of Pittsburgh |
| Schools and Programs: |
School of Medicine > Molecular Genetics and Developmental Biology |
| Degree: |
PhD - Doctor of Philosophy |
| Thesis Type: |
Doctoral Dissertation |
| Refereed: |
Yes |
| Uncontrolled Keywords: |
Spermatogonial stem cells, oncofertility |
| Date Deposited: |
04 May 2020 11:33 |
| Last Modified: |
04 May 2021 05:15 |
| URI: |
http://d-scholarship.pitt.edu/id/eprint/38779 |
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