Teng, Bo and Jiang, Junguang and Zhao, Lijing and Gao, Jing and Chen, Junyu and Liu, Zhe and Wang, Hongda and Lu, Binfeng
(2017)
Ginsenoside PPD’s Antitumor Effect via Down-Regulation of mTOR Revealed by Super-Resolution Imaging.
Molecules, 22 (3).
ISSN 1420-3049
Abstract
Derived from Panax ginseng, the natural product 20(S)-Protopanaxadiol (PPD) has been reported for its cytotoxicity against several cancer cell lines. The molecular mechanism is, however, not well understood. Here we show that PPD significantly inhibits proliferation, induces apoptosis and causes G2/M cell cycle arrest in human laryngeal carcinoma cells (Hep-2 cells). PPD also decreases the levels of proteins related to cell proliferation. Moreover, PPD-induced apoptosis is characterized by a dose-dependent down-regulation of Bcl-2 expression and up-regulation of Bax, and is accompanied by the activation of Caspase-3 as well. Further molecular mechanism is revealed by direct stochastic optical reconstruction microscopy (dSTORM)—a novel high-precision localization microscopy which enables effective resolution down to the order of 10 nm. It shows the expression and spatial arrangement of mTOR and its downstream effectors, demonstrating that this ginsenoside exerts its excellent anticancer effects via down-regulation of mTOR signaling pathway in Hep-2 cells. Taken together, our findings elucidate that the antitumor effect of PPD is associated with its regulation of mTOR expression and distribution, which encourages further studies of PPD as a promising therapeutic agent against laryngeal carcinoma.
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Details
| Item Type: |
Article
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| Status: |
Published |
| Creators/Authors: |
| Creators | Email | Pitt Username | ORCID  |
|---|
| Teng, Bo | | | | | Jiang, Junguang | | | | | Zhao, Lijing | | | | | Gao, Jing | | | | | Chen, Junyu | | | | | Liu, Zhe | | | | | Wang, Hongda | | | | | Lu, Binfeng | binfeng@pitt.edu | binfeng | |
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| Date: |
19 March 2017 |
| Date Type: |
Publication |
| Journal or Publication Title: |
Molecules |
| Volume: |
22 |
| Number: |
3 |
| Publisher: |
MDPI AG |
| DOI or Unique Handle: |
10.3390/molecules22030486 |
| Schools and Programs: |
School of Medicine > Immunology |
| Refereed: |
Yes |
| Uncontrolled Keywords: |
PPD, laryngeal cancer, mTOR, antitumor, dSTORM |
| ISSN: |
1420-3049 |
| Official URL: |
http://dx.doi.org/10.3390/molecules22030486 |
| Funders: |
National Nature Science Foundation of China, Program for International S&T Cooperation Projects of Jilin Province, Key Programs for Science and Technology Development of Jilin Province |
| Article Type: |
Research Article |
| Date Deposited: |
02 Feb 2021 18:14 |
| Last Modified: |
02 Feb 2021 18:14 |
| URI: |
http://d-scholarship.pitt.edu/id/eprint/40210 |
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