Demetris, AJ and Markus, BH and Saidman, S and Fung, JJ and Makowka, L and Graner, S and Duquesnoy, R and Starzl, TE
(1988)
Isolation and primary cultures of human intrahepatic bile ductular epithelium.
In Vitro Cellular & Developmental Biology, 24 (5).
464 - 470.
ISSN 0883-8364
Abstract
A technique for the isolation of human intrahepatic bile ductular epithelium, and the establishment of primary cultures using a serum- and growth-factor-supplemented medium combined with a connective tissue substrata is described. Initial cell isolates and monolayer cultures display phenotypic characteristics of biliary epithelial cells (low molecular weight prekeratin positive; albumin, alphafetoprotein, and Factor VIII-related antigen negative). Ultrastructural features of the cultured cells show cell polarization with surface microvilli, numerous interepithelial junctional complexes and cytoplasmic intermediate prekeratin filaments. © 1988 Tissue Culture Association, Inc.
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Item Type: |
Article
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Status: |
Published |
Creators/Authors: |
Creators | Email | Pitt Username | ORCID  |
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Demetris, AJ | | | | Markus, BH | | | | Saidman, S | | | | Fung, JJ | | | | Makowka, L | | | | Graner, S | | | | Duquesnoy, R | | | | Starzl, TE | tes11@pitt.edu | TES11 | |
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Centers: |
Other Centers, Institutes, Offices, or Units > Thomas E. Starzl Transplantation Institute |
Date: |
1 May 1988 |
Date Type: |
Publication |
Journal or Publication Title: |
In Vitro Cellular & Developmental Biology |
Volume: |
24 |
Number: |
5 |
Page Range: |
464 - 470 |
DOI or Unique Handle: |
10.1007/bf02628499 |
Institution: |
University of Pittsburgh |
Refereed: |
Yes |
ISSN: |
0883-8364 |
Other ID: |
uls-drl:31735062128685, Starzl CV No. 816 |
Date Deposited: |
08 Apr 2010 17:13 |
Last Modified: |
27 Jan 2019 02:55 |
URI: |
http://d-scholarship.pitt.edu/id/eprint/4202 |
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