Gifford, Emily
(2021)
Traumatic Brain Injury-related Cellular Metabolic Distress.
Master's Thesis, University of Pittsburgh.
(Unpublished)
Abstract
Traumatic Brain Injury is a devastating health crisis referred to as a “silent epidemic”, with an estimated 69 million people sustaining an injury each year, an injury which will likely have lasting effects. In the days and weeks following the primary insult to the brain, the lesion spreads, but there are limited strategies for tracking secondary injury, which is a pre-requisite to any preventions or treatments. Dexamethasone-enhanced microdialysis enables chemical monitoring in the brain for an extended length of time. Intracerebral microdialysis coupled with electrochemical detection is herein applied for monitoring and investigating glucose concentrations in the dialysate of humans as well as laboratory rat models of TBI. Recent results have documented a progressive decline and subsequent loss in measured levels of interstitial glucose near the injury site. We investigate the hypothesis that this extreme decrease in glucose in cortical dialysate reflects profound metabolic disruption in the tissue surrounding the microdialysis probe. This measured metabolic disruption could indicate the gradual spread of secondary injury, which would represent a significant advance in knowledge regarding the mechanism of spread as well as providing a clear marker of secondary injury that could eventually lead to intervention strategies.
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Details
| Item Type: |
University of Pittsburgh ETD
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| Status: |
Unpublished |
| Creators/Authors: |
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| ETD Committee: |
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| Date: |
18 December 2021 |
| Date Type: |
Publication |
| Defense Date: |
8 December 2021 |
| Approval Date: |
13 September 2024 |
| Submission Date: |
8 December 2021 |
| Access Restriction: |
No restriction; Release the ETD for access worldwide immediately. |
| Number of Pages: |
77 |
| Institution: |
University of Pittsburgh |
| Schools and Programs: |
Dietrich School of Arts and Sciences > Chemistry |
| Degree: |
MS - Master of Science |
| Thesis Type: |
Master's Thesis |
| Refereed: |
Yes |
| Uncontrolled Keywords: |
microdialysis, traumatic brain injury, biosensors, glucose decline, spreading depolarizations, continuous microdialysis, dexamethasone-enhanced microdialysis, metabolic distress |
| Date Deposited: |
13 Sep 2024 18:56 |
| Last Modified: |
13 Sep 2024 19:08 |
| URI: |
http://d-scholarship.pitt.edu/id/eprint/42074 |
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