Rossaro, L and Mazzaferro, V and Scotti‐Foglieni, CL and Williams, DS and Simplaceanu, E and Simplaceanu, V and Francavilla, A and Starzl, TE and Ho, C and Van Thiel, DH
(1991)
Effect of cyclosporine on hepatic energy status and on fructose metabolism after portacaval shunt in dog as monitored by phosphorus‐31 nuclear magnetic resonance spectroscopy in vivo.
Hepatology, 13 (4).
780 - 785.
ISSN 0270-9139
Abstract
The effect of cyclosporin A on the hepatic energy status and intracellular pH of the liver and its response to a fructose challenge has been investigated using in vivo phosphorus‐31 nuclear magnetic resonance spectroscopy in dogs. Three experimental groups were studied: (a) control dogs (n = 5), (b) dogs 4 days after the creation of an end‐to‐side portacaval shunt (n = 5), and (c) dogs 4 days after portacaval shunt and continuous infusion of cyclosporin A (4 mg/kg/day) by way of the left portal vein (portacaval shunt plus cyclosporin A, n = 5). The phosphorus‐31 nuclear magnetic resonance spectra were obtained at 81 MHz using a Bruker BIOSPEC II 4.7‐tesla nuclear magnetic resonance system equipped with a 40‐cm horizontal bore superconducting solenoid. The phosphomonoesters (p < 0.01), inorganic phosphate and ATP levels (p < 0.05) were decreased significantly in portacaval shunt–treated and in portacaval shunt‐pluscyclosporin A–treated dogs compared with unshunted control dogs. After a fructose challenge (750 mg/kg body wt, intravenously), fructose‐1‐phosphate metabolism was reduced in portacaval shunt–treated dogs compared with either the normal or portacaval shuntplus‐cyclosporin A–treated dogs (p < 0.05). Both portacaval shunt– and portacaval shunt‐plus‐cyclosporin A–treated dogs demonstrated a reduced decline in ATP levels after fructose infusion when compared with the controls (p < 0.05). Immediately after the fructose challenge, the intracellular pH decreased from 7.30 ± 0.03 to 7.00 ± 0.05 in all animals (p < 0.01) and then gradually returned to normal over 60 min. These data, obtained in vivo using phosphorus‐31 nuclear magnetic resonance spectroscopy of the liver after a portacaval shunt, suggest that: (a) the energy status of the liver is reduced in dogs with a portacaval shunt compared with that of normal controls and (b) cyclosporin A treatment ameliorates the reduction in hepatic metabolism normally observed after a fructose challenge to the liver with a portacaval shunt. (HEPATOLOGY 1991;13:780–785.) Copyright © 1991 American Association for the Study of Liver Diseases
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| Item Type: |
Article
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| Status: |
Published |
| Creators/Authors: |
| Creators | Email | Pitt Username | ORCID  |
|---|
| Rossaro, L | | | | | Mazzaferro, V | | | | | Scotti‐Foglieni, CL | | | | | Williams, DS | | | | | Simplaceanu, E | | | | | Simplaceanu, V | | | | | Francavilla, A | | | | | Starzl, TE | tes11@pitt.edu | TES11 | | | Ho, C | | | | | Van Thiel, DH | | | |
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| Centers: |
Other Centers, Institutes, Offices, or Units > Thomas E. Starzl Transplantation Institute |
| Date: |
1 January 1991 |
| Date Type: |
Publication |
| Journal or Publication Title: |
Hepatology |
| Volume: |
13 |
| Number: |
4 |
| Page Range: |
780 - 785 |
| DOI or Unique Handle: |
10.1002/hep.1840130427 |
| Institution: |
University of Pittsburgh |
| Refereed: |
Yes |
| ISSN: |
0270-9139 |
| Other ID: |
uls-drl:31735062117829, Starzl CV No. 1231 |
| Date Deposited: |
08 Apr 2010 17:21 |
| Last Modified: |
02 Aug 2020 14:56 |
| URI: |
http://d-scholarship.pitt.edu/id/eprint/4617 |
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