Irving, Dale
(2024)
Functional integration of bioengineered tooth roots in a rodent orthotopic implantation site: A Pilot Study.
Master's Thesis, University of Pittsburgh.
(Unpublished)
Abstract
In this study, we explore the orthotopic implantation of a scaffold free, tissue engineered tooth root organoid in a rat model as the first step in the replacement of titanium dental implants. Our lab has previously shown that postnatal dental pulp and periodontal ligament (PDL) stem/progenitor cells (DPSCs and PDLSCs, respectively) have the innate capacity to self-assemble to form tooth root organoids with 4 distinct tissues emulating the pulp, dentin, cementum, and
PDL. These organoids present a biomimetic model to study cellular dynamics driving dental tissue repair and could be utilized therapeutically as biological dental implants. As a first step towards this clinical translation, we aim to evaluate the capacity of these organoids to maintain their structure when implanted in vivo and assess their ability to integrate with native tissue structures. Using a 2-stage approach which is analogous to frequently employed clinical timelines, the maxillary right first molar of six nude rats was extracted atraumatically. The underlying bone was allowed 3 weeks to heal, at which time an osteotomy was created matching the approximate size of the organoids. These tissues were placed surgically to mimic the placement of a dental implant. Micro computed tomography analysis and histological analysis was performed on the samples at 2 and 4 weeks respectively. The organoid was not detected in the analysis of the samples; therefore, the surgical protocol was adjusted to a one stage approach. The final surgical protocol mimics an immediate implantation protocol where the same organoids were implanted at the time of tooth removal and stabilized with xenograft particulate or collagen and secured with a layer of cyanoacrylate adhesive dressing. Samples from the updated surgical protocol are being processed for analysis.
Share
| Citation/Export: |
|
| Social Networking: |
|
Details
| Item Type: |
University of Pittsburgh ETD
|
| Status: |
Unpublished |
| Creators/Authors: |
|
| ETD Committee: |
|
| Date: |
9 July 2024 |
| Date Type: |
Publication |
| Defense Date: |
6 June 2024 |
| Approval Date: |
9 July 2024 |
| Submission Date: |
7 June 2024 |
| Access Restriction: |
No restriction; Release the ETD for access worldwide immediately. |
| Number of Pages: |
40 |
| Institution: |
University of Pittsburgh |
| Schools and Programs: |
School of Dental Medicine > Dental Science |
| Degree: |
MDS - Master of Dental Science |
| Thesis Type: |
Master's Thesis |
| Refereed: |
Yes |
| Uncontrolled Keywords: |
Tooth Root Organoid Stem Cells |
| Date Deposited: |
09 Jul 2024 14:17 |
| Last Modified: |
09 Jul 2024 14:17 |
| URI: |
http://d-scholarship.pitt.edu/id/eprint/46497 |
Metrics
Monthly Views for the past 3 years
Plum Analytics
Actions (login required)
 |
View Item |
![[feed]](/images/feed-icon-32x32.png){kind=icon}