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Long-term survival of donor-specific pancreatic islet xenografts in fully xenogeneic chimeras (Wf rat → B10 mouse)

Zeng, Y and Ricordi, C and Tzakis, A and Rilo, HLR and Carroll, PB and Starzl, TE and Ildstad, ST (1992) Long-term survival of donor-specific pancreatic islet xenografts in fully xenogeneic chimeras (Wf rat → B10 mouse). Transplantation, 53 (2). 277 - 283. ISSN 0041-1337

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Abstract

We recently reported that reconstitution of lethally irradiated BIO mouse recipients with 40x10s untreated WF rat bone marrow cells resulted in stable fully xenogeneic chimerism (WF rat → B10 mouse). In these animals, the tolerance induced for skin xenografts was highly MHC specific in that donor-specific WF rat skin grafts were significantly prolonged while MHC-dispar-ate third-party xenografts were rapidly rejected (median survival time [MST] = 9 days). We have now examined whether islet cell xenografts placed under the renal capsule of chimeras rendered diabetic with strep-tozotocin would be accepted and remain functional to maintain euglycemia. Animals were prepared, typed for chimerism at 6 weeks, and diabetes induced with strep-tozotocin. Donor-specific WF (RtlA") islet cell xenografts were significantly prolonged (MST >180 days) in WF → B10 chimeras, while MHC-disparate third-party F344 rat (RtlA1) grafts were rejected with a time course similar to unmanipulated BIO mice (MST=8 days). The transplanted donor-specific islet cells were functional to maintain euglycemia, since removal of the grafts at from 100 to 180 days in selected individual chimeras uniformly resulted in return of the diabetic state. These data suggest that donor-specific islet cell xenografts are accepted and remain functional in mice rendered tolerant to rat xenoantigens following bone marrow transplantation. © 1992 by Williams and Wilkins.

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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Zeng, Y
Ricordi, C
Tzakis, A
Rilo, HLR
Carroll, PB
Starzl, TEtes11@pitt.eduTES11
Ildstad, ST
Centers: Other Centers, Institutes, Offices, or Units > Thomas E. Starzl Transplantation Institute
Date: 1 January 1992
Date Type: Publication
Journal or Publication Title: Transplantation
Volume: 53
Number: 2
Page Range: 277 - 283
DOI or Unique Handle: 10.1097/00007890-199202010-00005
Institution: University of Pittsburgh
Refereed: Yes
ISSN: 0041-1337
Other ID: uls-drl:31735062111343, Starzl CV No. 1341
Date Deposited: 08 Apr 2010 17:22
Last Modified: 19 Jul 2020 10:55
URI: http://d-scholarship.pitt.edu/id/eprint/4727

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