Sever, CE and Demetris, AJ and Zeng, J and Carroll, P and Tzakis, A and Fung, JJ and Starzl, TE and Ricordi, C
(1992)
Composition of human islet cell preparations for transplantation.
Acta Diabetologica, 28 (3-4).
233 - 238.
ISSN 0940-5429
Abstract
To study the cellular composition of human islet cell isolates for transplantation, formalin-fixed and paraffin-embedded cell pellets were stained by the immunoperoxidase method with a panel of antibodies characterising endocrine, epithelial, soft tissue and haematolymphoid components. Immediately after separation, the isolates contained 30-80% islet cells, differing mainly in the content of islet and acinar cells, whereas the soft tissue, ductal/ductular and haematolymphoid elements comprised a relatively constant 10-20%. After 1 week in culture the islet cell content of less highly purified isolates (30-40% islets) dropped dramatically to 5%. The highly purified isolates (70-80% islets) showed only a minimal change in cellular composition; however, approximately two-thirds of islet cells were degranulated and did not stain for insulin. Haematolymphoid components were still present in all cultured isolates. We conclude that primarily mechanical purification methods and short-term culture are not sufficient to eliminate highly immunogenic cells. In addition, short-term culture is deleterious to the isolate if a significant number of acinar cells is still present after enrichment. © 1992 Springer-Verlag.
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Item Type: |
Article
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Status: |
Published |
Creators/Authors: |
Creators | Email | Pitt Username | ORCID  |
---|
Sever, CE | | | | Demetris, AJ | | | | Zeng, J | | | | Carroll, P | | | | Tzakis, A | | | | Fung, JJ | | | | Starzl, TE | tes11@pitt.edu | TES11 | | Ricordi, C | | | |
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Centers: |
Other Centers, Institutes, Offices, or Units > Thomas E. Starzl Transplantation Institute |
Date: |
1 September 1992 |
Date Type: |
Publication |
Journal or Publication Title: |
Acta Diabetologica |
Volume: |
28 |
Number: |
3-4 |
Page Range: |
233 - 238 |
DOI or Unique Handle: |
10.1007/bf00779005 |
Institution: |
University of Pittsburgh |
Refereed: |
Yes |
ISSN: |
0940-5429 |
Other ID: |
uls-drl:31735062111632, Starzl CV No. 1370 |
Date Deposited: |
08 Apr 2010 17:23 |
Last Modified: |
27 Jan 2019 02:55 |
URI: |
http://d-scholarship.pitt.edu/id/eprint/4756 |
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