Link to the University of Pittsburgh Homepage
Link to the University Library System Homepage Link to the Contact Us Form

IMPROVING BIOCOMPATIBILITY AND CHRONIC PERFORMANCE OF NEURAL PROBES USING SURFACE IMMOBILIZATION OF THE NEURAL ADHESION MOLECULE L1

Azemi, Erdrin (2010) IMPROVING BIOCOMPATIBILITY AND CHRONIC PERFORMANCE OF NEURAL PROBES USING SURFACE IMMOBILIZATION OF THE NEURAL ADHESION MOLECULE L1. Doctoral Dissertation, University of Pittsburgh. (Unpublished)

[img]
Preview
PDF
Primary Text

Download (8MB) | Preview

Abstract

Neural interface technologies that link the nervous system and the outside world by either stimulating or recording from neural tissue, show great promise for patients suffering from various neurological injuries or disorders. However, the poor recording stability and longevity of neural interface devices (neural probes) is an imminent obstacle to their advance in widespread clinical applications. The dominant factor that affects chronic neural recordings has been reported to be the inflammatory tissue response including neuronal loss and gliosis at the electrode/tissue interface. In this study, we proposed to modify the surface of neural probes with the neural adhesion molecule L1. The L1 molecule is known to specifically promote neurite outgrowth and neuronal survival. We hypothesized that surface immobilization of L1, may introduce a neuron friendly environment to maintain healthy neuronal density and promote neurite outgrowth around the recording electrodes. Consequently, this phenomenon could reduce gliosis formation. Silane chemistry and the heterobifunctional coupling agent, 4-Maleimidobutyric acid N-hydroxysuccinimide ester (GMBS), were used to covalently bind L1 onto the silicon surface. Polyethylene glycol (PEG)-NH2 was co-immobilized to cap unreacted GMBS groups and prevent non-specific cell attachment. Primary murine neurons and astrocytes were cultured on L1 modified and control surfaces. The L1 surfaces showed promoted neuronal attachment and neurite outgrowth but significantly reduced astrocyte attachment relative to controls. L1 vs. non modified control probes were implanted in the rat motor cortex for 1, 4, and 8 weeks. Extensive immunohistochemistry and quantitative image analysis were performed to assess the brain tissue response to implants. The results showed that the L1 modified probes had no loss of neurons around the implant interface and showed a significant increase of axonal density compared to the control at all time points. Additionally, significantly reduced glia cell activation and recruitment was observed at the vicinity of the L1 modified probes. As a final step, we have developed a method to evaluate the chronic recording performance of neural probes in the rat somatosensory cortex from whisker stimulation and cortical recordings. Based on our results we conclude that the L1 biomolecule shows neuroprotective and neurogenerative properties while inhibiting gliosis. The L1 surface coating can be a promising strategy to improve the biocompatibility of all types of neural probes and their chronic performance in the brain.


Share

Citation/Export:
Social Networking:
Share |

Details

Item Type: University of Pittsburgh ETD
Status: Unpublished
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Azemi, Erdrinerdrin.azemi@gmail.com
ETD Committee:
TitleMemberEmail AddressPitt UsernameORCID
Committee ChairCui, Xinyan Tracyxic11@pitt.eduXIC11
Committee MemberLagenaur, Carllagenaur@pitt.eduLAGENAUR
Committee MemberWeber, Douglasdjw50@pitt.eduDJW50
Committee MemberMarra, Kaceymarrkx@upmc.edu
Committee MemberWatkins, Simonswatkins@pitt.eduSWATKINS
Date: 25 June 2010
Date Type: Completion
Defense Date: 25 March 2010
Approval Date: 25 June 2010
Submission Date: 12 April 2010
Access Restriction: 5 year -- Restrict access to University of Pittsburgh for a period of 5 years.
Institution: University of Pittsburgh
Schools and Programs: Swanson School of Engineering > Bioengineering
Degree: PhD - Doctor of Philosophy
Thesis Type: Doctoral Dissertation
Refereed: Yes
Uncontrolled Keywords: biocompatibility; gliosis; L1; neural probes; neural recording
Other ID: http://etd.library.pitt.edu/ETD/available/etd-04122010-134858/, etd-04122010-134858
Date Deposited: 10 Nov 2011 19:36
Last Modified: 15 Nov 2016 13:39
URI: http://d-scholarship.pitt.edu/id/eprint/7068

Metrics

Monthly Views for the past 3 years

Plum Analytics


Actions (login required)

View Item View Item