Tataurov, Andrey V
(2005)
MEASUREMENT OF LATERAL DIFFUSION IN LIPIDS.
Master's Thesis, University of Pittsburgh.
(Unpublished)
Abstract
Different techniques to study lateral diffusion of lipids are reviewed, including long-range diffusion methods and short-range diffusion methods. Long-range diffusion methods require the measurements of the time needed to fill a given area of the membrane. Applications of fluorescence recovery after photobleaching (FRAP) method are discussed. Short-range diffusion methods measure the rate of bimolecular collisions. The methods include fluorescence quenching, fluorescence energy transfer, nuclear magnetic resonance and electron spin resonance spectroscopy. Special attention is given to Electron Spin Resonance (ESR) spectroscopy. Procedure of obtaining diffusion coefficient D using novel two dimensional electron double-double resonance experiment (2D-ELDOR) is discussed in greater detail. In order to demonstrate the feasibility of using 2D Fourier Transform ESR spectroscopy for lateral diffusion studies, the D of spin-labeled phospholipids sample is estimated by 2D-ELDOR method.
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Details
Item Type: |
University of Pittsburgh ETD
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Status: |
Unpublished |
Creators/Authors: |
Creators | Email | Pitt Username | ORCID  |
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Tataurov, Andrey V | avt1@pitt.edu | AVT1 | |
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ETD Committee: |
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Date: |
9 June 2005 |
Date Type: |
Completion |
Defense Date: |
19 April 2005 |
Approval Date: |
9 June 2005 |
Submission Date: |
20 April 2005 |
Access Restriction: |
No restriction; Release the ETD for access worldwide immediately. |
Institution: |
University of Pittsburgh |
Schools and Programs: |
Dietrich School of Arts and Sciences > Chemistry |
Degree: |
MS - Master of Science |
Thesis Type: |
Master's Thesis |
Refereed: |
Yes |
Uncontrolled Keywords: |
2D-ELDOR; diffusion coefficient; EPR; ESR; lateral diffusion; lipids |
Other ID: |
http://etd.library.pitt.edu/ETD/available/etd-04202005-224253/, etd-04202005-224253 |
Date Deposited: |
10 Nov 2011 19:39 |
Last Modified: |
15 Nov 2016 13:41 |
URI: |
http://d-scholarship.pitt.edu/id/eprint/7347 |
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