Dai, XiaoHu
(2007)
Purifacation of Wild Type EcoRI Endonuclease and EcoRI Endonuclease RS187Crystal Growth of WT EcoRI Endonuclease-DNA comlex and EcoRI Endonuclease.
Master's Thesis, University of Pittsburgh.
(Unpublished)
Abstract
EcoRI endonuclease is a very useful tool to study the structural mechanism of protein-DNA recognition. In this work, wild type EcoRI Endonuclease and EcoRI Endonuclease mutant RS187 were purified to high purity. Crystals of wild type EcoRI Endonuclease-DNA 13mer complex have been obtained with good size and shape. Some small crystals of EcoRI Endonuclease RS187-DNA 13mer complex were also grown.
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Details
| Item Type: |
University of Pittsburgh ETD
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| Status: |
Unpublished |
| Creators/Authors: |
|
| ETD Committee: |
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| Date: |
19 September 2007 |
| Date Type: |
Completion |
| Defense Date: |
30 May 2007 |
| Approval Date: |
19 September 2007 |
| Submission Date: |
11 June 2007 |
| Access Restriction: |
No restriction; Release the ETD for access worldwide immediately. |
| Institution: |
University of Pittsburgh |
| Schools and Programs: |
Dietrich School of Arts and Sciences > Molecular Biophysics |
| Degree: |
MS - Master of Science |
| Thesis Type: |
Master's Thesis |
| Refereed: |
Yes |
| Uncontrolled Keywords: |
DNA; EcoRI endonuclease |
| Other ID: |
http://etd.library.pitt.edu/ETD/available/etd-06112007-125605/, etd-06112007-125605 |
| Date Deposited: |
10 Nov 2011 19:46 |
| Last Modified: |
19 Dec 2016 14:36 |
| URI: |
http://d-scholarship.pitt.edu/id/eprint/8073 |
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