Data for: Aging-induced weakness of mouse NMJs is associated with reduced active zone density, synaptic event kinetics, and presynaptic calcium entry

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  • Data for: Aging-induced weakness of mouse NMJs is associated with reduced active zone density, synaptic event kinetics, and presynaptic calcium entry
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  • 04/29/2026
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  • Aging has been shown to affect both the structure and function of the neuromuscular junction. In our previous study, we documented a biphasic change (first an increase followed by a decrease) in neurotransmission over the aging time course at male mouse neuromuscular junctions (NMJs). Here, we explored several potential mechanisms behind the reduction in presynaptic neurotransmitter release in the later stages of aging. We found that the active zone (AZ) density significantly decreased in NMJs at 30 months of age, compared to 4- and 26-month-old mice. Further, the decreased end plate potential (EPP) amplitude in these 30-month-old mice was associated with a significantly longer rise and decay time, and while miniature end plate potential (mEPPs) amplitude and frequency were unchanged, these events also had a longer rise and decay time. 30-month-old NMJs also showed a significant reduction in the vesicle replenishment rate (VRR). Additionally, 30 to 33-month-old NMJs had reduced presynaptic calcium entry following a single presynaptic action potential. Taken together, these transmitter release site changes may explain age-induced reductions in neuromuscular neurotransmission in aged mice and may also lead to the identification of novel therapeutic targets or potential biomarkers for future research in this area.
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MLA citation style (9th ed.)

Li, Yizhi, et al. Data For: Aging-induced Weakness of Mouse Nmjs Is Associated with Reduced Active Zone Density, Synaptic Event Kinetics, and Presynaptic Calcium Entry. University of Pittsburgh. 2026. d-scholarship.pitt.edu/concern/generic_works/3a50fae7-3f8a-49aa-bd87-6a906b5fe45d.

APA citation style (7th ed.)

L. Yizhi, C. Elinor, B. Chris, M. Anna, G. Meera, J. Anousha, B. Yomna, & M. Stephen. (2026). Data for: Aging-induced weakness of mouse NMJs is associated with reduced active zone density, synaptic event kinetics, and presynaptic calcium entry. https://d-scholarship.pitt.edu/concern/generic_works/3a50fae7-3f8a-49aa-bd87-6a906b5fe45d

Chicago citation style (CMOS 17, author-date)

Li, Yizhi, Case, Elinor, Blanchard, Chris, Monteleone, Anna, Gandhi, Meera, Jaie, Anousha, Badawi, Yomna et al. Data For: Aging-Induced Weakness of Mouse Nmjs Is Associated with Reduced Active Zone Density, Synaptic Event Kinetics, and Presynaptic Calcium Entry. University of Pittsburgh. 2026. https://d-scholarship.pitt.edu/concern/generic_works/3a50fae7-3f8a-49aa-bd87-6a906b5fe45d.

Note: These citations are programmatically generated and may be incomplete.

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