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Genetic analysis of peptidoglycan biosynthesis in mycobacteria: Characterization of a ddlA mutant of Mycobacterium smegmatis

Belanger, AE and Porter, JC and Hatfull, GF (2000) Genetic analysis of peptidoglycan biosynthesis in mycobacteria: Characterization of a ddlA mutant of Mycobacterium smegmatis. Journal of Bacteriology, 182 (23). 6854 - 6856. ISSN 0021-9193

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Abstract

A temperature-sensitive mutant of Mycobacterium smegmatis was characterized that contains a mutation in ddlA, the gene encoding D-alanine:D-alanine ligase. Enzymatic assays using recombinant proteins and D-cycloserine susceptibility indicate that the A365V mutation in the SMEG35 DdlA protein causes a reduction in enzymatic activity in vitro and in vivo.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Belanger, AE
Porter, JC
Hatfull, GFgfh@pitt.eduGFH
Date: 1 December 2000
Date Type: Publication
Journal or Publication Title: Journal of Bacteriology
Volume: 182
Number: 23
Page Range: 6854 - 6856
DOI or Unique Handle: 10.1128/jb.182.23.6854-6856.2000
Schools and Programs: Dietrich School of Arts and Sciences > Biological Sciences
Refereed: Yes
ISSN: 0021-9193
MeSH Headings: Base Sequence; DNA, Bacterial; Gene Expression; Molecular Sequence Data; Mutagenesis; Mycobacterium smegmatis--enzymology; Mycobacterium smegmatis--genetics; Peptide Synthases--genetics; Peptide Synthases--metabolism; Peptidoglycan--biosynthesis; Temperature
Other ID: NLM PMC111435
PubMed Central ID: PMC111435
PubMed ID: 11073937
Date Deposited: 12 Nov 2012 15:52
Last Modified: 02 Feb 2019 15:57
URI: http://d-scholarship.pitt.edu/id/eprint/16125

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