Link to the University of Pittsburgh Homepage
Link to the University Library System Homepage Link to the Contact Us Form

The degradation of glial scar and enhancement of chronic intracortical recording electrode performance through the local delivery of dexamethasone and chondroitinase

Alba, Nicolas A (2014) The degradation of glial scar and enhancement of chronic intracortical recording electrode performance through the local delivery of dexamethasone and chondroitinase. Doctoral Dissertation, University of Pittsburgh. (Unpublished)

Primary Text

Download (6MB) | Preview


The ability of conducting polymers such as poly(3,4-ethylenedioxythiophene) (PEDOT) to store a drug as a dopant and release it following electrical stimulus make them an intriguing coating possibility for intracortical electrodes, along with their ability to reduce electrode impedance. The mechanism allows for the release of an assortment of useful agents, including anti-inflammatory drugs and neuromodulatory chemicals. We evaluated the release capabilities of a multi-walled carbon nanotube (MWCNT)-doped PEDOT coating incorporating the anti-inflammatory steroid dexamethasone in vitro using sputtered-gold macroelectrodes, and then applied the coating to half of the electrodes within 16-shank platinum/iridium floating microelectrode arrays for chronic in vivo evaluation in rat visual cortex. Impedance measurement, neurophysiological recording, and cyclic voltammetric release stimulus (-0.9 V to 0.6 V, 1 V/s, 20 cycles) was performed daily to all channels. On the 11th day, histology was performed to quantitatively characterize inflammatory tissue response using OX42 (microglia) and GFAP (astroglia). Equivalent circuit analysis was performed to assist the interpretation of impedance data. Our results indicated that the MWCNT/PEDOT-coated gold macroelectrodes released double the amount of dexamethasone using passive release followed by CV stimulation (10 sets of 20 cycles) compared to passive release alone. Coatings applied to Pt/Ir microelectrodes reduced 1 kHz impedance in PBS by approximately 38%. Coated probes in vivo exhibited a significant decrease in 1 kHz impedance for the initial three days of implantation followed by an increase, between days 4 and 7, to values equivalent to those exhibited by uncoated probes. Neurophysiological recording performance of coated and uncoated probes remained equivalent for the duration of the experiment, in terms of signal-to-noise ratio and noise amplitude. Histology revealed no significant difference in tissue inflammatory response to coated and uncoated electrodes. Explant imaging revealed the presence of a membranous film enveloping coated electrodes, and equivalent circuit analysis suggested that the day 4-7 increase in 1 kHz impedance of coated electrodes was due to a decrease in effective surface area of the coatings as well as the core electrodes. Additional work was also performed developing a model for the in vivo microinjection of the enzyme Chondroitinase ABC into tissue surrounding implanted microelectrodes.


Social Networking:
Share |


Item Type: University of Pittsburgh ETD
Status: Unpublished
CreatorsEmailPitt UsernameORCID
Alba, Nicolas Ania16@pitt.eduNIA16
ETD Committee:
TitleMemberEmail AddressPitt UsernameORCID
Committee ChairCui, Xinyan Tracyxic11@pitt.eduXIC11
Committee MemberWeber, Douglas Jdjw50@pitt.eduDJW50
Committee MemberLittle, Steven Rsrlittle@pitt.eduSRLITTLE
Committee MemberLagenaur, Carl Flagenaur@pitt.eduLAGENAUR
Committee MemberHalfter, Williwhalfter@pitt.eduWHALFTER
Date: 29 January 2014
Date Type: Publication
Defense Date: 3 April 2013
Approval Date: 29 January 2014
Submission Date: 2 December 2013
Access Restriction: 2 year -- Restrict access to University of Pittsburgh for a period of 2 years.
Number of Pages: 151
Institution: University of Pittsburgh
Schools and Programs: Swanson School of Engineering > Bioengineering
Degree: PhD - Doctor of Philosophy
Thesis Type: Doctoral Dissertation
Refereed: Yes
Uncontrolled Keywords: neural interface, conducting polymers, drug release, surface engineering
Date Deposited: 29 Jan 2014 16:46
Last Modified: 15 Nov 2016 14:16


Monthly Views for the past 3 years

Plum Analytics

Actions (login required)

View Item View Item