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Isolation and characterization of human anterior cruciate ligament-derived vascular stem cells

Matsumoto, T and Ingham, SM and Mifune, Y and Osawa, A and Logar, A and Usas, A and Kuroda, R and Kurosaka, M and Fu, FH and Huard, J (2012) Isolation and characterization of human anterior cruciate ligament-derived vascular stem cells. Stem Cells and Development, 21 (6). 859 - 872. ISSN 1547-3287

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The anterior cruciate ligament (ACL) usually fails to heal after rupture mainly due to the inability of the cells within the ACL tissue to establish an adequate healing process, making graft reconstruction surgery a necessity. However, some reports have shown that there is a healing potential of ACL with primary suture repair. Although some reports showed the existence of mesenchymal stem cell-like cells in human ACL tissues, their origin still remains unclear. Recently, blood vessels have been reported to represent a rich supply of stem/progenitor cells with a characteristic expression of CD34 and CD146. In this study, we attempted to validate the hypothesis that CD34- and CD146-expressing vascular cells exist in hACL tissues, have a potential for multi-lineage differentiation, and are recruited to the rupture site to participate in the intrinsic healing of injured ACL. Immunohistochemistry and flow cytometry analysis of hACL tissues demonstrated that it contains significantly more CD34 and CD146-positive cells in the ACL ruptured site compared with the noninjured midsubstance. CD34+CD45- cells isolated from ACL ruptured site showed higher expansionary potentials than CD146+CD45- and CD34-CD146-CD45- cells, and displayed higher differentiation potentials into osteogenic, adipogenic, and angiogenic lineages than the other cell populations. Immunohistochemistry of fetal and adult hACL tissues demonstrated a higher number of CD34 and CD146-positive cells in the ACL septum region compared with the midsubstance. In conclusion, our findings suggest that the ACL septum region contains a population of vascular-derived stem cells that may contribute to ligament regeneration and repair at the site of rupture. © 2012 Mary Ann Liebert, Inc.


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Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
Matsumoto, T
Ingham, SM
Mifune, Y
Osawa, A
Logar, A
Usas, A
Kuroda, R
Kurosaka, M
Fu, FHffu@pitt.eduFFU
Huard, J
Centers: Other Centers, Institutes, Offices, or Units > Stem Cell Research Center
Date: 10 April 2012
Date Type: Publication
Journal or Publication Title: Stem Cells and Development
Volume: 21
Number: 6
Page Range: 859 - 872
DOI or Unique Handle: 10.1089/scd.2010.0528
Schools and Programs: School of Medicine > Biochemistry and Molecular Genetics
School of Medicine > Orthopaedic Surgery
Swanson School of Engineering > Bioengineering
Refereed: Yes
ISSN: 1547-3287
MeSH Headings: Anterior Cruciate Ligament--blood supply; Antigens, CD146--analysis; Antigens, CD34--analysis; Blood Vessels--cytology; Cell Differentiation; Flow Cytometry; Humans; Immunohistochemistry; Stem Cells--cytology; Wound Healing
Other ID: NLM PMC3871494
PubMed Central ID: PMC3871494
PubMed ID: 21732814
Date Deposited: 04 Apr 2014 15:41
Last Modified: 20 Jan 2019 10:55


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