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Comparative genomics of Cluster O mycobacteriophages

Cresawn, SG and Pope, WH and Jacobs-Sera, D and Bowman, CA and Russell, DA and Dedrick, RM and Adair, T and Anders, KR and Ball, S and Bollivar, D and Breitenberger, C and Burnett, SH and Butela, K and Byrnes, D and Carzo, S and Cornely, KA and Cross, T and Daniels, RL and Dunbar, D and Findley, AM and Gissendanner, CR and Golebiewska, UP and Hartzog, GA and Hatherill, JR and Hughes, LE and Jalloh, CS and De Los Santos, C and Ekanem, K and Khambule, SL and King, RA and King-Smith, C and Klyczek, K and Krukonis, GP and Laing, C and Lapin, JS and Lopez, AJ and Mkhwanazi, SM and Molloy, SD and Moran, D and Munsamy, V and Pacey, E and Plymale, R and Poxleitner, M and Reyna, N and Schildbach, JF and Stukey, J and Taylor, SE and Ware, VC and Wellmann, AL and Westholm, D and Wodarski, D and Zajko, M and Zikalala, TS and Hendrix, RW and Hatfull, GF (2015) Comparative genomics of Cluster O mycobacteriophages. PLoS ONE, 10 (3).

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Mycobacteriophages - viruses of mycobacterial hosts - are genetically diverse but morphologically are all classified in the Caudovirales with double-stranded DNA and tails. We describe here a group of five closely related mycobacteriophages - Corndog, Catdawg, Dylan, Firecracker, and YungJamal - designated as Cluster O with long flexible tails but with unusual prolate capsids. Proteomic analysis of phage Corndog particles, Catdawg particles, and Corndog-infected cells confirms expression of half of the predicted gene products and indicates a non-canonical mechanism for translation of the Corndog tape measure protein. Bioinformatic analysis identifies 8-9 strongly predicted SigA promoters and all five Cluster O genomes contain more than 30 copies of a 17 bp repeat sequence with dyad symmetry located throughout the genomes. Comparison of the Cluster O phages provides insights into phage genome evolution including the processes of gene flux by horizontal genetic exchange.


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Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
Cresawn, SG
Pope, WH
Jacobs-Sera, Ddjs@pitt.eduDJS0000-0001-7838-3257
Bowman, CA
Russell, DAdar78@pitt.eduDAR780000-0001-6894-2748
Dedrick, RMdedrick@pitt.eduDEDRICK
Adair, T
Anders, KR
Ball, S
Bollivar, D
Breitenberger, C
Burnett, SH
Butela, KKAB340@pitt.eduKAB340
Byrnes, D
Carzo, S
Cornely, KA
Cross, T
Daniels, RL
Dunbar, D
Findley, AM
Gissendanner, CR
Golebiewska, UP
Hartzog, GA
Hatherill, JR
Hughes, LE
Jalloh, CS
De Los Santos, C
Ekanem, K
Khambule, SL
King, RA
King-Smith, C
Klyczek, K
Krukonis, GP
Laing, C
Lapin, JS
Lopez, AJ
Mkhwanazi, SM
Molloy, SD
Moran, D
Munsamy, V
Pacey, E
Plymale, R
Poxleitner, M
Reyna, N
Schildbach, JF
Stukey, J
Taylor, SE
Ware, VC
Wellmann, AL
Westholm, D
Wodarski, D
Zajko, M
Zikalala, TS
Hendrix, RWrhx@pitt.eduRHX
Hatfull, GFgfh@pitt.eduGFH
ContributionContributors NameEmailPitt UsernameORCID
Date: 5 March 2015
Date Type: Publication
Journal or Publication Title: PLoS ONE
Volume: 10
Number: 3
DOI or Unique Handle: 10.1371/journal.pone.0118725
Schools and Programs: Dietrich School of Arts and Sciences > Biological Sciences
Refereed: Yes
Article Type: Review
PubMed ID: 25742016
Date Deposited: 12 May 2015 18:06
Last Modified: 30 Mar 2021 10:55


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