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Role of Inorganic Phosphate in Vesicle Release from Osteogenic Cells

Smethurst, Victoria (2018) Role of Inorganic Phosphate in Vesicle Release from Osteogenic Cells. Master's Thesis, University of Pittsburgh. (Unpublished)

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Abstract

Extracellular vesicles (EV) are membrane-enclosed microstructures released from the cell. EV can transport cargo—such as DNA, RNA, and proteins—into the extracellular space and act as a form of intercellular communication. Matrix vesicles (MV) are a unique type of EV produced by mineralizing cells. In previous studies, utilizing in vitro models of pre-odontoblasts and vascular smooth muscle cells, it was determined that phosphate induces the release of MV and alters MV composition. We hypothesized that elevated phosphate alters the composition of MV through the alteration of gene expression. To determine whether this hypothesis holds true or not, 17IIA11 cells, a pre-odontoblast cell line, were exposed to elevated phosphate for 24h, 48h, and 72h. RNA and MV were then collected for further analysis. Using nanoparticle tracking analysis, we determined that the size distribution of the MV was not altered by the phosphate. Next, to evaluate a possible mechanism driving MV composition change, we compared the effect of phosphate on gene expression to the effect of phosphate on MV composition. No correlation was identified between changes in gene expression and changes in MV composition. These findings suggest that phosphate does not alter the composition of MV through altered gene expression. Future studies should focus on other mechanisms that may drive MV composition changes.


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Details

Item Type: University of Pittsburgh ETD
Status: Unpublished
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Smethurst, Victoriaves33@pitt.eduves33
ETD Committee:
TitleMemberEmail AddressPitt UsernameORCID
Committee ChairNapierala, Dobrawadon11@pitt.eduDon11
Committee MemberBeniash, Eliasebeniash@pitt.eduebeniash
Committee MemberTaboas, Juanjmt106@pitt.edujmt106
Date: 29 August 2018
Date Type: Publication
Defense Date: 19 July 2018
Approval Date: 29 August 2018
Submission Date: 17 August 2018
Access Restriction: 2 year -- Restrict access to University of Pittsburgh for a period of 2 years.
Number of Pages: 59
Institution: University of Pittsburgh
Schools and Programs: School of Dental Medicine > Dental Science
Degree: MS - Master of Science
Thesis Type: Master's Thesis
Refereed: Yes
Uncontrolled Keywords: N/A
Date Deposited: 29 Aug 2018 14:37
Last Modified: 29 Aug 2018 14:37
URI: http://d-scholarship.pitt.edu/id/eprint/35252

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  • Role of Inorganic Phosphate in Vesicle Release from Osteogenic Cells. (deposited 29 Aug 2018 14:37) [Currently Displayed]

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