Link to the University of Pittsburgh Homepage
Link to the University Library System Homepage Link to the Contact Us Form

Bone regeneration mediated by BMP4-expressing muscle-derived stem cells is affected by delivery system

Huard, J and Usas, A and Ho, AM and Cooper, GM and Olshanski, A and Peng, H (2009) Bone regeneration mediated by BMP4-expressing muscle-derived stem cells is affected by delivery system. Tissue Engineering - Part A, 15 (2). 285 - 293. ISSN 1937-3341

[img]
Preview
PDF
Published Version
Available under License : See the attached license file.

Download (460kB) | Preview
[img] Plain Text (licence)
Available under License : See the attached license file.

Download (1kB)

Abstract

This study investigated the delivery of bone morphogenetic protein (BMP)4-secreting muscle-derived stem cells (MDSC-B4) capable of inducing bone formation in mice using collagen gel (CG), fibrin sealant (FS), and gelatin sponge carriers. After implanting these various cell-loaded scaffolds intramuscularly or into critical-size skull defects, we measured the extent of heterotopic ossification and calvarial defect healing over a 6-week period via radiographic, radiomorphometric, histological, and micro-computed tomography analyses. As expected, in the absence of MDSC-B4, there was no ectopic ossification and only minimal calvarial regeneration using each type of scaffold. Although CG and gelatin sponges loaded with BMP4-secreting cells produced the most ectopic bone, FS constructs produced bone with comparably less mineralization. In the mouse calvaria, we observed MDSC-B4-loaded scaffolds able to promote bone defect healing to a variable degree, but there were differences between these implants in the volume, shape, and morphology of regenerated bone. MDSC-B4 delivery in a gelatin sponge produced hypertrophic bone, whereas delivery in a CG and FS healed the defect with bone that closely resembled the quantity and configuration of native calvarium. In summary, hydrogels are suitable carriers for osteocompetent MDSCs in promoting bone regeneration, especially at craniofacial injury sites. © 2009, Mary Ann Liebert, Inc.


Share

Citation/Export:
Social Networking:
Share |

Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Huard, J
Usas, A
Ho, AM
Cooper, GMgmc8@pitt.eduGMC8
Olshanski, A
Peng, H
Centers: Other Centers, Institutes, Offices, or Units > Stem Cell Research Center
Date: 1 February 2009
Date Type: Publication
Journal or Publication Title: Tissue Engineering - Part A
Volume: 15
Number: 2
Page Range: 285 - 293
DOI or Unique Handle: 10.1089/ten.tea.2008.0130
Schools and Programs: School of Medicine > Orthopaedic Surgery
Refereed: Yes
ISSN: 1937-3341
MeSH Headings: Animals; Bone Morphogenetic Protein 4--metabolism; Bone Regeneration--physiology; Choristoma--pathology; Drug Delivery Systems; Mice; Muscles--cytology; Organ Size; Osteogenesis; Prosthesis Implantation; Skull--pathology; Skull--radiography; Stem Cells--cytology; Stem Cells--metabolism; Wound Healing; X-Ray Microtomography
Other ID: NLM PMC2810214
PubMed Central ID: PMC2810214
PubMed ID: 19061430
Date Deposited: 09 Apr 2014 16:40
Last Modified: 02 Feb 2019 14:55
URI: http://d-scholarship.pitt.edu/id/eprint/21047

Metrics

Monthly Views for the past 3 years

Plum Analytics

Altmetric.com


Actions (login required)

View Item View Item