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A gene expression profile test to resolve head & neck squamous versus lung squamous cancers

Lal, A and Panos, R and Marjanovic, M and Walker, M and Fuentes, E and Kubicek, GJ and Henner, WD and Buturovic, LJ and Halks-Miller, M (2013) A gene expression profile test to resolve head & neck squamous versus lung squamous cancers. Diagnostic Pathology, 8 (1).

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Abstract

Background: The differential diagnosis between metastatic head & neck squamous cell carcinomas (HNSCC) and lung squamous cell carcinomas (lung SCC) is often unresolved because the histologic appearance of these two tumor types is similar. We have developed and validated a gene expression profile test (GEP-HN-LS) that distinguishes HNSCC and lung SCC in formalin-fixed, paraffin-embedded (FFPE) specimens using a 2160-gene classification model.Methods: The test was validated in a blinded study using a pre-specified algorithm and microarray data files for 76 metastatic or poorly-differentiated primary tumors with a known HNSCC or lung SCC diagnosis.Results: The study met the primary Bayesian statistical endpoint for acceptance. Measures of test performance include overall agreement with the known diagnosis of 82.9% (95% CI, 72.5% to 90.6%), an area under the ROC curve (AUC) of 0.91 and a diagnostics odds ratio (DOR) of 23.6. HNSCC (N = 38) gave an agreement with the known diagnosis of 81.6% and lung SCC (N = 38) gave an agreement of 84.2%. Reproducibility in test results between three laboratories had a concordance of 91.7%.Conclusion: GEP-HN-LS can aid in resolving the important differential diagnosis between HNSCC and lung SCC tumors.Virtual Slides: The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1753227817890930. © 2013 Lal et al.; licensee BioMed Central Ltd.

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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Lal, A
Panos, R
Marjanovic, M
Walker, M
Fuentes, E
Kubicek, GJ
Henner, WD
Buturovic, LJ
Halks-Miller, M
Date: 11 March 2013
Date Type: Publication
Journal or Publication Title: Diagnostic Pathology
Volume: 8
Number: 1
DOI or Unique Handle: 10.1186/1746-1596-8-44
Schools and Programs: School of Medicine > Radiation Oncology
Refereed: Yes
Date Deposited: 06 Oct 2016 19:47
Last Modified: 20 Dec 2018 00:55
URI: http://d-scholarship.pitt.edu/id/eprint/29749

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